6PCM

Crystal Structure of Mycobacterium smegmatis Topoisomerase I with ssDNA bound to both N- and C-terminal domains

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003916	molecular_function	DNA topoisomerase activity
A	0003917	molecular_function	DNA topoisomerase type I (single strand cut, ATP-independent) activity
A	0006265	biological_process	DNA topological change
A	0016853	molecular_function	isomerase activity
A	0046872	molecular_function	metal ion binding
B	0003677	molecular_function	DNA binding
B	0003916	molecular_function	DNA topoisomerase activity
B	0003917	molecular_function	DNA topoisomerase type I (single strand cut, ATP-independent) activity
B	0006265	biological_process	DNA topological change
B	0016853	molecular_function	isomerase activity
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue SO4 A 901

Chain	Residue
A	ARG373
A	GLN374

---

site_id	AC2
Number of Residues	2
Details	binding site for residue SO4 A 902

Chain	Residue
A	VAL134
A	ARG151

---

site_id	AC3
Number of Residues	2
Details	binding site for residue SO4 A 903

Chain	Residue
A	TYR32
A	ASP394

---

site_id	AC4
Number of Residues	3
Details	binding site for residue SO4 A 904

Chain	Residue
A	THR250
A	GLY251
A	ARG252

---

site_id	AC5
Number of Residues	2
Details	binding site for residue SO4 A 905

Chain	Residue
A	THR821
A	GLU822

---

site_id	AC6
Number of Residues	2
Details	binding site for residue SO4 A 906

Chain	Residue
A	ARG207
A	PRO508

---

site_id	AC7
Number of Residues	4
Details	binding site for residue SO4 A 907

Chain	Residue
A	PHE319
A	SER320
A	GLU514
A	LYS547

---

site_id	AC8
Number of Residues	3
Details	binding site for residue SO4 A 908

Chain	Residue
A	ARG653
A	ARG656
A	GLU662

---

site_id	AC9
Number of Residues	3
Details	binding site for residue SO4 A 909

Chain	Residue
A	GLN674
B	ARG653
B	ARG678

---

site_id	AD1
Number of Residues	2
Details	binding site for residue SO4 B 901

Chain	Residue
B	ARG51
D	DG17

---

site_id	AD2
Number of Residues	3
Details	binding site for residue SO4 B 902

Chain	Residue
B	ARG207
B	SER507
B	PRO508

---

site_id	AD3
Number of Residues	2
Details	binding site for residue SO4 B 903

Chain	Residue
B	SER320
B	ARG323

---

site_id	AD4
Number of Residues	1
Details	binding site for residue SO4 B 904

Chain	Residue
B	GLN361

---

site_id	AD5
Number of Residues	2
Details	binding site for residue SO4 B 905

Chain	Residue
B	GLY393
B	ASP394

---

site_id	AD6
Number of Residues	1
Details	binding site for residue SO4 B 906

Chain	Residue
B	GLY549

---

site_id	AD7
Number of Residues	4
Details	binding site for residue SO4 B 907

Chain	Residue
B	ARG571
B	ASP574
B	ASP576
B	PHE577

---

site_id	AD8
Number of Residues	3
Details	binding site for residue SO4 B 908

Chain	Residue
B	PRO57
B	ALA58
B	LYS59

---

site_id	AD9
Number of Residues	3
Details	binding site for residue SO4 C 101

Chain	Residue
C	DC8
C	DG9
D	DG5

---

site_id	AE1
Number of Residues	4
Details	binding site for residue SO4 C 102

Chain	Residue
A	ARG51
C	DC15
C	DC16
C	DG17

---

site_id	AE2
Number of Residues	3
Details	binding site for residue SO4 D 101

Chain	Residue
C	DG5
D	DC8
D	DG9

---

site_id	AE3
Number of Residues	1
Details	binding site for residue SO4 D 102

Chain	Residue
D	DG21

---

Functional Information from PROSITE/UniProt

site_id	PS00287
Number of Residues	14
Details	CYSTATIN Cysteine proteases inhibitors signature. KQLVGGNLEgIDAR

Chain	Residue	Details
A	LYS621-ARG634

---

site_id	PS00396
Number of Residues	15
Details	TOPO_IA_1 Topoisomerase (Topo) IA-type active site signature. QrLYEngy.........ITYmRTD

Chain	Residue	Details
A	GLN329-ASP343

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	248
Details	Domain: {"description":"Toprim","evidences":[{"source":"HAMAP-Rule","id":"MF_00952","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	Region: {"description":"Interaction with DNA","evidences":[{"source":"HAMAP-Rule","id":"MF_00952","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	27
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Active site: {"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU01383","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"9593741","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00952","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	16
Details	Site: {"description":"Interaction with DNA","evidences":[{"source":"HAMAP-Rule","id":"MF_00952","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---