6PCM
Crystal Structure of Mycobacterium smegmatis Topoisomerase I with ssDNA bound to both N- and C-terminal domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-03 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 133.325, 135.067, 145.087 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.442 - 3.107 |
| R-factor | 0.2279 |
| Rwork | 0.225 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cqi |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.443 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.073 | 0.849 |
| Rmeas | 0.083 | 0.938 |
| Rpim | 0.037 | 0.391 |
| Number of reflections | 47334 | 2351 |
| <I/σ(I)> | 29.9 | 1.7 |
| Completeness [%] | 98.9 | 99.5 |
| Redundancy | 4.9 | 5.4 |
| CC(1/2) | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris:HCl, 1.5M lithium Sulfate |
