6HYT

Crystal structure of DHX8 helicase domain bound to ADP at 2.3 Angstrom

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003676	molecular_function	nucleic acid binding
A	0003724	molecular_function	RNA helicase activity
A	0005524	molecular_function	ATP binding
B	0000398	biological_process	mRNA splicing, via spliceosome
B	0003676	molecular_function	nucleic acid binding
B	0003724	molecular_function	RNA helicase activity
B	0005524	molecular_function	ATP binding
C	0000398	biological_process	mRNA splicing, via spliceosome
C	0003676	molecular_function	nucleic acid binding
C	0003724	molecular_function	RNA helicase activity
C	0005524	molecular_function	ATP binding
D	0000398	biological_process	mRNA splicing, via spliceosome
D	0003676	molecular_function	nucleic acid binding
D	0003724	molecular_function	RNA helicase activity
D	0005524	molecular_function	ATP binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue ADP A 1301

Chain	Residue
A	THR590
A	PHE825
A	THR847
A	ASP849
A	ARG893
A	MG1302
A	HOH1405
A	HOH1436
A	HOH1455
A	HOH1465
A	GLY591
A	SER592
A	GLY593
A	LYS594
A	THR595
A	THR596
A	SER625
A	ARG629

---

site_id	AC2
Number of Residues	6
Details	binding site for residue MG A 1302

Chain	Residue
A	THR595
A	ADP1301
A	HOH1405
A	HOH1436
A	HOH1465
A	HOH1493

---

site_id	AC3
Number of Residues	5
Details	binding site for residue EDO A 1303

Chain	Residue
A	PHE1018
A	LYS1032
A	ASP1041
A	THR1044
A	GLU1164

---

site_id	AC4
Number of Residues	4
Details	binding site for residue EDO A 1304

Chain	Residue
A	PRO805
A	SER832
A	HOH1422
A	HOH1433

---

site_id	AC5
Number of Residues	2
Details	binding site for residue EDO A 1305

Chain	Residue
A	HIS1136
A	ARG1163

---

site_id	AC6
Number of Residues	2
Details	binding site for residue DMS A 1306

Chain	Residue
A	THR736
A	HOH1411

---

site_id	AC7
Number of Residues	2
Details	binding site for residue DMS A 1308

Chain	Residue
A	ALA605
A	GLY606

---

site_id	AC8
Number of Residues	3
Details	binding site for residue ACT A 1309

Chain	Residue
A	THR749
A	THR763
A	ARG901

---

site_id	AC9
Number of Residues	2
Details	binding site for residue CL A 1310

Chain	Residue
A	TYR568
A	LYS571

---

site_id	AD1
Number of Residues	18
Details	binding site for residue ADP B 1301

Chain	Residue
B	THR590
B	GLY591
B	SER592
B	GLY593
B	LYS594
B	THR595
B	THR596
B	SER625
B	ARG629
B	PHE825
B	THR847
B	ASP849
B	ARG893
B	MG1302
B	HOH1404
B	HOH1415
B	HOH1427
B	HOH1485

---

site_id	AD2
Number of Residues	7
Details	binding site for residue MG B 1302

Chain	Residue
B	THR595
B	ASP685
B	ADP1301
B	HOH1404
B	HOH1415
B	HOH1427
B	HOH1492

---

site_id	AD3
Number of Residues	4
Details	binding site for residue EDO B 1303

Chain	Residue
B	HIS768
B	PRO805
B	HOH1422
C	PRO896

---

site_id	AD4
Number of Residues	5
Details	binding site for residue EDO B 1304

Chain	Residue
B	TYR742
B	THR894
B	GLY895
C	PRO773
C	ARG833

---

site_id	AD5
Number of Residues	5
Details	binding site for residue EDO B 1305

Chain	Residue
B	PHE1018
B	LYS1032
B	ASP1041
B	THR1044
B	GLU1164

---

site_id	AD6
Number of Residues	2
Details	binding site for residue DMS B 1306

Chain	Residue
B	GLN766
B	GLU771

---

site_id	AD7
Number of Residues	1
Details	binding site for residue DMS B 1307

Chain	Residue
B	ARG1163

---

site_id	AD8
Number of Residues	1
Details	binding site for residue DMS B 1308

Chain	Residue
B	THR736

---

site_id	AD9
Number of Residues	1
Details	binding site for residue DMS B 1309

Chain	Residue
B	ASP968

---

site_id	AE1
Number of Residues	2
Details	binding site for residue DMS B 1310

Chain	Residue
B	ALA605
B	GLY606

---

site_id	AE2
Number of Residues	1
Details	binding site for residue DMS B 1311

Chain	Residue
B	GLY858

---

site_id	AE3
Number of Residues	1
Details	binding site for residue DMS B 1312

Chain	Residue
B	GLN1104

---

site_id	AE4
Number of Residues	2
Details	binding site for residue CL B 1314

Chain	Residue
B	TYR568
B	LYS571

---

site_id	AE5
Number of Residues	17
Details	binding site for residue ADP C 1301

Chain	Residue
C	THR595
C	THR596
C	SER625
C	ARG629
C	PHE825
C	THR847
C	ASP849
C	MG1302
C	HOH1406
C	HOH1408
C	HOH1430
C	HOH1432
C	THR590
C	GLY591
C	SER592
C	GLY593
C	LYS594

---

site_id	AE6
Number of Residues	6
Details	binding site for residue MG C 1302

Chain	Residue
C	THR595
C	ADP1301
C	HOH1406
C	HOH1408
C	HOH1430
C	HOH1480

---

site_id	AE7
Number of Residues	5
Details	binding site for residue EDO C 1303

Chain	Residue
C	THR645
C	ILE646
C	PHE648
C	GLU649
C	MET665

---

site_id	AE8
Number of Residues	5
Details	binding site for residue EDO C 1304

Chain	Residue
C	PHE1018
C	LYS1032
C	ASP1041
C	THR1044
C	GLU1164

---

site_id	AE9
Number of Residues	2
Details	binding site for residue DMS C 1306

Chain	Residue
C	PHE735
C	THR736

---

site_id	AF1
Number of Residues	2
Details	binding site for residue DMS C 1307

Chain	Residue
C	GLN1104
C	TRP1172

---

site_id	AF2
Number of Residues	5
Details	binding site for residue DMS C 1308

Chain	Residue
C	GLN640
C	CYS651
C	THR652
D	GLU649
D	DMS1305

---

site_id	AF3
Number of Residues	2
Details	binding site for residue CL C 1309

Chain	Residue
C	TYR568
C	LYS571

---

site_id	AF4
Number of Residues	20
Details	binding site for residue ADP D 1301

Chain	Residue
D	LEU565
D	THR590
D	GLY591
D	SER592
D	GLY593
D	LYS594
D	THR595
D	THR596
D	SER625
D	ARG629
D	PHE825
D	THR847
D	ASP849
D	ARG893
D	MG1302
D	HOH1407
D	HOH1408
D	HOH1427
D	HOH1443
D	HOH1447

---

site_id	AF5
Number of Residues	6
Details	binding site for residue MG D 1302

Chain	Residue
D	THR595
D	ADP1301
D	HOH1407
D	HOH1408
D	HOH1447
D	HOH1501

---

site_id	AF6
Number of Residues	7
Details	binding site for residue EDO D 1303

Chain	Residue
D	PHE1018
D	LYS1032
D	ASP1041
D	THR1044
D	LEU1045
D	ALA1140
D	GLU1164

---

site_id	AF7
Number of Residues	5
Details	binding site for residue EDO D 1304

Chain	Residue
A	TYR742
A	PRO896
D	PRO773
D	SER832
D	ARG833

---

site_id	AF8
Number of Residues	4
Details	binding site for residue DMS D 1305

Chain	Residue
C	GLU649
C	DMS1308
D	CYS651
D	THR652

---

site_id	AF9
Number of Residues	3
Details	binding site for residue DMS D 1306

Chain	Residue
D	GLN766
D	THR770
D	TYR900

---

site_id	AG1
Number of Residues	2
Details	binding site for residue DMS D 1307

Chain	Residue
D	PHE735
D	THR736

---

site_id	AG2
Number of Residues	2
Details	binding site for residue DMS D 1308

Chain	Residue
D	HIS1136
D	ARG1163

---

site_id	AG3
Number of Residues	2
Details	binding site for residue CL D 1309

Chain	Residue
D	TYR568
D	LYS571

---

Functional Information from PROSITE/UniProt

site_id	PS00690
Number of Residues	10
Details	DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. AiIMLDEAHE

Chain	Residue	Details
A	ALA680-GLU689

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	489
Details	Domain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	720
Details	Domain: {"description":"Helicase C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00542","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	9
Details	Motif: {"description":"DEAH box"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	28
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---