6HYT
Crystal structure of DHX8 helicase domain bound to ADP at 2.3 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97242 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.947, 167.576, 137.231 |
Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
Resolution | 33.800 - 2.330 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.250 | 2.370 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.088 | 1.554 |
Number of reflections | 127918 | |
<I/σ(I)> | 8.9 | 0.9 |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 3.4 | 3.2 |
CC(1/2) | 0.998 | 0.293 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% 2-Methyl-2,4-pentanediol, 100 mM Sodium Acetate, 6%DMSO |