6GKM

CryoEM structure of the MDA5-dsRNA filament in complex with ATP (10 mM)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002376	biological_process	immune system process
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0003724	molecular_function	RNA helicase activity
A	0003725	molecular_function	double-stranded RNA binding
A	0003727	molecular_function	single-stranded RNA binding
A	0004386	molecular_function	helicase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0008270	molecular_function	zinc ion binding
A	0009615	biological_process	response to virus
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0016925	biological_process	protein sumoylation
A	0019904	molecular_function	protein domain specific binding
A	0032727	biological_process	positive regulation of interferon-alpha production
A	0032728	biological_process	positive regulation of interferon-beta production
A	0032755	biological_process	positive regulation of interleukin-6 production
A	0032760	biological_process	positive regulation of tumor necrosis factor production
A	0038187	molecular_function	pattern recognition receptor activity
A	0039530	biological_process	MDA-5 signaling pathway
A	0042802	molecular_function	identical protein binding
A	0043021	molecular_function	ribonucleoprotein complex binding
A	0045071	biological_process	negative regulation of viral genome replication
A	0045087	biological_process	innate immune response
A	0046872	molecular_function	metal ion binding
A	0051259	biological_process	protein complex oligomerization
A	0051607	biological_process	defense response to virus
A	0060337	biological_process	type I interferon-mediated signaling pathway
A	0060760	biological_process	positive regulation of response to cytokine stimulus
A	0071360	biological_process	cellular response to exogenous dsRNA
A	0098586	biological_process	cellular response to virus
A	0140374	biological_process	antiviral innate immune response

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 1101

Chain	Residue
A	CYS907
A	CYS910
A	CYS962
A	CYS964

---

site_id	AC2
Number of Residues	7
Details	binding site for residue ATP A 1102

Chain	Residue
A	THR337
A	ARG338
A	ARG310
A	GLY333
A	SER334
A	GLY335
A	LYS336

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01125

Chain	Residue	Details
A	LYS925
A	HIS928
A	PRO980
A	LEU982

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	SITE: Cleavage

Chain	Residue	Details
A	ASP208
A	ASP216
A	ASP251

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21183079

Chain	Residue	Details
A	SER289
A	SER291
A	SER302

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17242355, ECO:0007744|PubMed:21183079

Chain	Residue	Details
A	SER645
A	SER666

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine; by RIOK3 => ECO:0000250|UniProtKB:Q9BYX4

Chain	Residue	Details
A	VAL846

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) => ECO:0000250|UniProtKB:Q9BYX4

Chain	Residue	Details
A	LYS23
A	LYS43

---