6GKM
CryoEM structure of the MDA5-dsRNA filament in complex with ATP (10 mM)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.87 |
| Number of Particle | 526596 |
| Software | RELION,PHENIX,EPU,Gctf,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
