Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5NG6

Crystal structure of FnCas12a bound to a crRNA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004530	molecular_function	deoxyribonuclease I activity
A	0006259	biological_process	DNA metabolic process
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0051607	biological_process	defense response to virus
C	0003677	molecular_function	DNA binding
C	0003723	molecular_function	RNA binding
C	0004518	molecular_function	nuclease activity
C	0004519	molecular_function	endonuclease activity
C	0004530	molecular_function	deoxyribonuclease I activity
C	0006259	biological_process	DNA metabolic process
C	0016787	molecular_function	hydrolase activity
C	0016829	molecular_function	lyase activity
C	0051607	biological_process	defense response to virus
E	0003677	molecular_function	DNA binding
E	0003723	molecular_function	RNA binding
E	0004518	molecular_function	nuclease activity
E	0004519	molecular_function	endonuclease activity
E	0004530	molecular_function	deoxyribonuclease I activity
E	0006259	biological_process	DNA metabolic process
E	0016787	molecular_function	hydrolase activity
E	0016829	molecular_function	lyase activity
E	0051607	biological_process	defense response to virus
G	0003677	molecular_function	DNA binding
G	0003723	molecular_function	RNA binding
G	0004518	molecular_function	nuclease activity
G	0004519	molecular_function	endonuclease activity
G	0004530	molecular_function	deoxyribonuclease I activity
G	0006259	biological_process	DNA metabolic process
G	0016787	molecular_function	hydrolase activity
G	0016829	molecular_function	lyase activity
G	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue MG A 1401

Chain	Residue
A	ARG800
A	HOH1502
A	HOH1504
B	A-3
B	U-4
B	HOH302
B	HOH305

site_id	AC2
Number of Residues	6
Details	binding site for residue MG B 200

Chain	Residue
B	HOH307
B	HOH308
B	HOH309
B	HOH310
A	HOH1505
B	HOH306

site_id	AC3
Number of Residues	3
Details	binding site for residue MG C 1401

Chain	Residue
C	ARG800
D	A-3
D	U-4

site_id	AC4
Number of Residues	6
Details	binding site for residue MG D 200

Chain	Residue
D	HOH301
D	HOH302
D	HOH303
D	HOH304
D	HOH305
D	HOH306

site_id	AC5
Number of Residues	6
Details	binding site for residue MG E 1401

Chain	Residue
E	ARG800
E	HOH1502
E	HOH1505
F	A-3
F	HOH303
F	HOH308

site_id	AC6
Number of Residues	6
Details	binding site for residue MG F 200

Chain	Residue
F	HOH302
F	HOH304
F	HOH306
F	HOH307
F	HOH309
F	HOH310

site_id	AC7
Number of Residues	5
Details	binding site for residue MG G 1401

Chain	Residue
G	ARG800
G	HOH1501
G	HOH1502
H	A-3
H	HOH302

site_id	AC8
Number of Residues	6
Details	binding site for residue MG H 200

Chain	Residue
G	HOH1503
H	HOH301
H	HOH303
H	HOH304
H	HOH305
H	HOH306

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	32
Details	Region: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	16
Details	Region: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	12
Details	Region: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	12
Details	Region: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	32
Details	Region: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	280
Details	Region: {"description":"Wedge region 2","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	68
Details	Region: {"description":"LKL, important for PAM recognition and DNA unwinding","evidences":[{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	396
Details	Region: {"description":"PAM-interacting domain (PI)","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	24
Details	Region: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	48
Details	Region: {"description":"Binds single-strand non-target DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	516
Details	Region: {"description":"Wedge region 3","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	40
Details	Region: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	240
Details	Region: {"description":"RuvC-I","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	424
Details	Region: {"description":"RuvC-II","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	12
Details	Active site: {"description":"For pre-crRNA processing","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	12
Details	Active site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"26422227","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	4
Details	Site: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	4
Details	Site: {"description":"Binds target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	4
Details	Site: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	8
Details	Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	4
Details	Site: {"description":"Caps the crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	4
Details	Site: {"description":"Binds DNA protospacer adjacent motif (PAM)","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI23
Number of Residues	4
Details	Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI24
Number of Residues	4
Details	Site: {"description":"Binds PAM","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI25
Number of Residues	8
Details	Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI26
Number of Residues	4
Details	Site: {"description":"Binds Target strand DNA; via amide nitrogen","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI27
Number of Residues	4
Details	Site: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI28
Number of Residues	4
Details	Site: {"description":"Stabilizes transition state for pre-crRNA processing","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI29
Number of Residues	8
Details	Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)