5NG6
Crystal structure of FnCas12a bound to a crRNA
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004530 | molecular_function | deoxyribonuclease I activity |
| A | 0006259 | biological_process | DNA metabolic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0033898 | molecular_function | Bacillus subtilis ribonuclease activity |
| A | 0051607 | biological_process | defense response to virus |
| C | 0003677 | molecular_function | DNA binding |
| C | 0003723 | molecular_function | RNA binding |
| C | 0004519 | molecular_function | endonuclease activity |
| C | 0004530 | molecular_function | deoxyribonuclease I activity |
| C | 0006259 | biological_process | DNA metabolic process |
| C | 0016829 | molecular_function | lyase activity |
| C | 0033898 | molecular_function | Bacillus subtilis ribonuclease activity |
| C | 0051607 | biological_process | defense response to virus |
| E | 0003677 | molecular_function | DNA binding |
| E | 0003723 | molecular_function | RNA binding |
| E | 0004519 | molecular_function | endonuclease activity |
| E | 0004530 | molecular_function | deoxyribonuclease I activity |
| E | 0006259 | biological_process | DNA metabolic process |
| E | 0016829 | molecular_function | lyase activity |
| E | 0033898 | molecular_function | Bacillus subtilis ribonuclease activity |
| E | 0051607 | biological_process | defense response to virus |
| G | 0003677 | molecular_function | DNA binding |
| G | 0003723 | molecular_function | RNA binding |
| G | 0004519 | molecular_function | endonuclease activity |
| G | 0004530 | molecular_function | deoxyribonuclease I activity |
| G | 0006259 | biological_process | DNA metabolic process |
| G | 0016829 | molecular_function | lyase activity |
| G | 0033898 | molecular_function | Bacillus subtilis ribonuclease activity |
| G | 0051607 | biological_process | defense response to virus |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | binding site for residue MG A 1401 |
| Chain | Residue |
| A | ARG800 |
| A | HOH1502 |
| A | HOH1504 |
| B | A-3 |
| B | U-4 |
| B | HOH302 |
| B | HOH305 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue MG B 200 |
| Chain | Residue |
| B | HOH307 |
| B | HOH308 |
| B | HOH309 |
| B | HOH310 |
| A | HOH1505 |
| B | HOH306 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue MG C 1401 |
| Chain | Residue |
| C | ARG800 |
| D | A-3 |
| D | U-4 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue MG D 200 |
| Chain | Residue |
| D | HOH301 |
| D | HOH302 |
| D | HOH303 |
| D | HOH304 |
| D | HOH305 |
| D | HOH306 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue MG E 1401 |
| Chain | Residue |
| E | ARG800 |
| E | HOH1502 |
| E | HOH1505 |
| F | A-3 |
| F | HOH303 |
| F | HOH308 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue MG F 200 |
| Chain | Residue |
| F | HOH302 |
| F | HOH304 |
| F | HOH306 |
| F | HOH307 |
| F | HOH309 |
| F | HOH310 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue MG G 1401 |
| Chain | Residue |
| G | ARG800 |
| G | HOH1501 |
| G | HOH1502 |
| H | A-3 |
| H | HOH302 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue MG H 200 |
| Chain | Residue |
| G | HOH1503 |
| H | HOH301 |
| H | HOH303 |
| H | HOH304 |
| H | HOH305 |
| H | HOH306 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 12 |
| Details | ACT_SITE: For pre-crRNA processing => ECO:0000305|PubMed:28431230 |
| Chain | Residue | Details |
| A | HIS843 | |
| G | HIS843 | |
| G | LYS852 | |
| G | LYS869 | |
| A | LYS852 | |
| A | LYS869 | |
| C | HIS843 | |
| C | LYS852 | |
| C | LYS869 | |
| E | HIS843 | |
| E | LYS852 | |
| E | LYS869 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | ACT_SITE: For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 |
| Chain | Residue | Details |
| A | ASP917 | |
| G | ASP917 | |
| G | GLU1006 | |
| G | ASP1255 | |
| A | GLU1006 | |
| A | ASP1255 | |
| C | ASP917 | |
| C | GLU1006 | |
| C | ASP1255 | |
| E | ASP917 | |
| E | GLU1006 | |
| E | ASP1255 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | SITE: Binds crRNA alone and in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | THR16 | |
| C | THR16 | |
| E | THR16 | |
| G | THR16 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | SITE: Binds target strand DNA => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | LYS131 | |
| C | LYS131 | |
| E | LYS131 | |
| G | LYS131 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | SITE: Binds crRNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | THR295 | |
| C | THR295 | |
| E | THR295 | |
| G | THR295 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 12 |
| Details | SITE: Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | LYS320 | |
| G | LYS320 | |
| G | SER334 | |
| G | LYS589 | |
| A | SER334 | |
| A | LYS589 | |
| C | LYS320 | |
| C | SER334 | |
| C | LYS589 | |
| E | LYS320 | |
| E | SER334 | |
| E | LYS589 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | SITE: Caps the crRNA-target DNA heteroduplex => ECO:0000305|PubMed:28431230 |
| Chain | Residue | Details |
| A | TYR410 | |
| C | TYR410 | |
| E | TYR410 | |
| G | TYR410 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | SITE: Binds DNA protospacer adjacent motif (PAM) => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | LYS613 | |
| C | LYS613 | |
| E | LYS613 | |
| G | LYS613 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 4 |
| Details | SITE: Binds Target strand DNA => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | LYS667 | |
| C | LYS667 | |
| E | LYS667 | |
| G | LYS667 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | SITE: Binds PAM => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | LYS671 | |
| C | LYS671 | |
| E | LYS671 | |
| G | LYS671 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 8 |
| Details | SITE: Binds Target strand DNA => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | LYS677 | |
| A | LYS823 | |
| C | LYS677 | |
| C | LYS823 | |
| E | LYS677 | |
| E | LYS823 | |
| G | LYS677 | |
| G | LYS823 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 4 |
| Details | SITE: Binds Target strand DNA; via amide nitrogen => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | GLY826 | |
| C | GLY826 | |
| E | GLY826 | |
| G | GLY826 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 4 |
| Details | SITE: Binds crRNA => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | ARG833 | |
| C | ARG833 | |
| E | ARG833 | |
| G | ARG833 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 4 |
| Details | SITE: Stabilizes transition state for pre-crRNA processing => ECO:0000305|PubMed:28431230 |
| Chain | Residue | Details |
| A | LYS852 | |
| C | LYS852 | |
| E | LYS852 | |
| G | LYS852 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 8 |
| Details | SITE: Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | LYS1026 | |
| A | THR1063 | |
| C | LYS1026 | |
| C | THR1063 | |
| E | LYS1026 | |
| E | THR1063 | |
| G | LYS1026 | |
| G | THR1063 |
