5NG6
Crystal structure of FnCas12a bound to a crRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-26 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00768 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 214.684, 277.061, 135.636 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.239 - 3.342 |
R-factor | 0.2397 |
Rwork | 0.239 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5id6 |
RMSD bond length | 0.012 |
RMSD bond angle | 0.687 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 169.700 | 3.540 |
High resolution limit [Å] | 3.340 | 3.340 |
Number of reflections | 225590 | 33814 |
<I/σ(I)> | 10.9 | 1.73 |
Completeness [%] | 99.7 | 98.1 |
Redundancy | 7 | 7 |
CC(1/2) | 0.997 | 0.595 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50 mM HEPES-NaOH, pH 6.5, 20% (w/v) Polyethylene glycol 3,400, 1.5% (w/v) Tryptone |