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5FI0

Crystal Structure of the P-Rex1 DH/PH tandem in complex with Rac1

Functional Information from GO Data
ChainGOidnamespacecontents
A0005085molecular_functionguanyl-nucleotide exchange factor activity
A0035556biological_processintracellular signal transduction
B0001764biological_processneuron migration
B0001934biological_processpositive regulation of protein phosphorylation
B0003376biological_processsphingosine-1-phosphate receptor signaling pathway
B0003924molecular_functionGTPase activity
B0003925molecular_functionG protein activity
B0005515molecular_functionprotein binding
B0005525molecular_functionGTP binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005789cellular_componentendoplasmic reticulum membrane
B0005802cellular_componenttrans-Golgi network
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005884cellular_componentactin filament
B0005886cellular_componentplasma membrane
B0005925cellular_componentfocal adhesion
B0005938cellular_componentcell cortex
B0006954biological_processinflammatory response
B0007015biological_processactin filament organization
B0007155biological_processcell adhesion
B0007160biological_processcell-matrix adhesion
B0007163biological_processestablishment or maintenance of cell polarity
B0007264biological_processsmall GTPase-mediated signal transduction
B0008045biological_processmotor neuron axon guidance
B0008360biological_processregulation of cell shape
B0008361biological_processregulation of cell size
B0009611biological_processresponse to wounding
B0009653biological_processanatomical structure morphogenesis
B0010310biological_processregulation of hydrogen peroxide metabolic process
B0010591biological_processregulation of lamellipodium assembly
B0010592biological_processpositive regulation of lamellipodium assembly
B0010595biological_processpositive regulation of endothelial cell migration
B0010764biological_processnegative regulation of fibroblast migration
B0010811biological_processpositive regulation of cell-substrate adhesion
B0016020cellular_componentmembrane
B0016477biological_processcell migration
B0016601biological_processRac protein signal transduction
B0016787molecular_functionhydrolase activity
B0019899molecular_functionenzyme binding
B0019901molecular_functionprotein kinase binding
B0030027cellular_componentlamellipodium
B0030031biological_processcell projection assembly
B0030032biological_processlamellipodium assembly
B0030036biological_processactin cytoskeleton organization
B0030041biological_processactin filament polymerization
B0030334biological_processregulation of cell migration
B0030425cellular_componentdendrite
B0030667cellular_componentsecretory granule membrane
B0030865biological_processcortical cytoskeleton organization
B0031116biological_processpositive regulation of microtubule polymerization
B0031410cellular_componentcytoplasmic vesicle
B0031529biological_processruffle organization
B0031996molecular_functionthioesterase binding
B0032587cellular_componentruffle membrane
B0032707biological_processnegative regulation of interleukin-23 production
B0032956biological_processregulation of actin cytoskeleton organization
B0034446biological_processsubstrate adhesion-dependent cell spreading
B0035025biological_processpositive regulation of Rho protein signal transduction
B0035556biological_processintracellular signal transduction
B0036464cellular_componentcytoplasmic ribonucleoprotein granule
B0042470cellular_componentmelanosome
B0042995cellular_componentcell projection
B0043020cellular_componentNADPH oxidase complex
B0043197cellular_componentdendritic spine
B0043652biological_processengulfment of apoptotic cell
B0044877molecular_functionprotein-containing complex binding
B0045202cellular_componentsynapse
B0045428biological_processregulation of nitric oxide biosynthetic process
B0045730biological_processrespiratory burst
B0048012biological_processhepatocyte growth factor receptor signaling pathway
B0048261biological_processnegative regulation of receptor-mediated endocytosis
B0048870biological_processcell motility
B0051022molecular_functionRho GDP-dissociation inhibitor binding
B0051492biological_processregulation of stress fiber assembly
B0051496biological_processpositive regulation of stress fiber assembly
B0051668biological_processlocalization within membrane
B0051894biological_processpositive regulation of focal adhesion assembly
B0055038cellular_componentrecycling endosome membrane
B0060071biological_processWnt signaling pathway, planar cell polarity pathway
B0060263biological_processregulation of respiratory burst
B0060326biological_processcell chemotaxis
B0070062cellular_componentextracellular exosome
B0071526biological_processsemaphorin-plexin signaling pathway
B0090023biological_processpositive regulation of neutrophil chemotaxis
B0097178biological_processruffle assembly
B0098794cellular_componentpostsynapse
B0098978cellular_componentglutamatergic synapse
B0101003cellular_componentficolin-1-rich granule membrane
B1900026biological_processpositive regulation of substrate adhesion-dependent cell spreading
B1902622biological_processregulation of neutrophil migration
C0005085molecular_functionguanyl-nucleotide exchange factor activity
C0035556biological_processintracellular signal transduction
D0001764biological_processneuron migration
D0001934biological_processpositive regulation of protein phosphorylation
D0003376biological_processsphingosine-1-phosphate receptor signaling pathway
D0003924molecular_functionGTPase activity
D0003925molecular_functionG protein activity
D0005515molecular_functionprotein binding
D0005525molecular_functionGTP binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005789cellular_componentendoplasmic reticulum membrane
D0005802cellular_componenttrans-Golgi network
D0005829cellular_componentcytosol
D0005856cellular_componentcytoskeleton
D0005884cellular_componentactin filament
D0005886cellular_componentplasma membrane
D0005925cellular_componentfocal adhesion
D0005938cellular_componentcell cortex
D0006954biological_processinflammatory response
D0007015biological_processactin filament organization
D0007155biological_processcell adhesion
D0007160biological_processcell-matrix adhesion
D0007163biological_processestablishment or maintenance of cell polarity
D0007264biological_processsmall GTPase-mediated signal transduction
D0008045biological_processmotor neuron axon guidance
D0008360biological_processregulation of cell shape
D0008361biological_processregulation of cell size
D0009611biological_processresponse to wounding
D0009653biological_processanatomical structure morphogenesis
D0010310biological_processregulation of hydrogen peroxide metabolic process
D0010591biological_processregulation of lamellipodium assembly
D0010592biological_processpositive regulation of lamellipodium assembly
D0010595biological_processpositive regulation of endothelial cell migration
D0010764biological_processnegative regulation of fibroblast migration
D0010811biological_processpositive regulation of cell-substrate adhesion
D0016020cellular_componentmembrane
D0016477biological_processcell migration
D0016601biological_processRac protein signal transduction
D0016787molecular_functionhydrolase activity
D0019899molecular_functionenzyme binding
D0019901molecular_functionprotein kinase binding
D0030027cellular_componentlamellipodium
D0030031biological_processcell projection assembly
D0030032biological_processlamellipodium assembly
D0030036biological_processactin cytoskeleton organization
D0030041biological_processactin filament polymerization
D0030334biological_processregulation of cell migration
D0030425cellular_componentdendrite
D0030667cellular_componentsecretory granule membrane
D0030865biological_processcortical cytoskeleton organization
D0031116biological_processpositive regulation of microtubule polymerization
D0031410cellular_componentcytoplasmic vesicle
D0031529biological_processruffle organization
D0031996molecular_functionthioesterase binding
D0032587cellular_componentruffle membrane
D0032707biological_processnegative regulation of interleukin-23 production
D0032956biological_processregulation of actin cytoskeleton organization
D0034446biological_processsubstrate adhesion-dependent cell spreading
D0035025biological_processpositive regulation of Rho protein signal transduction
D0035556biological_processintracellular signal transduction
D0036464cellular_componentcytoplasmic ribonucleoprotein granule
D0042470cellular_componentmelanosome
D0042995cellular_componentcell projection
D0043020cellular_componentNADPH oxidase complex
D0043197cellular_componentdendritic spine
D0043652biological_processengulfment of apoptotic cell
D0044877molecular_functionprotein-containing complex binding
D0045202cellular_componentsynapse
D0045428biological_processregulation of nitric oxide biosynthetic process
D0045730biological_processrespiratory burst
D0048012biological_processhepatocyte growth factor receptor signaling pathway
D0048261biological_processnegative regulation of receptor-mediated endocytosis
D0048870biological_processcell motility
D0051022molecular_functionRho GDP-dissociation inhibitor binding
D0051492biological_processregulation of stress fiber assembly
D0051496biological_processpositive regulation of stress fiber assembly
D0051668biological_processlocalization within membrane
D0051894biological_processpositive regulation of focal adhesion assembly
D0055038cellular_componentrecycling endosome membrane
D0060071biological_processWnt signaling pathway, planar cell polarity pathway
D0060263biological_processregulation of respiratory burst
D0060326biological_processcell chemotaxis
D0070062cellular_componentextracellular exosome
D0071526biological_processsemaphorin-plexin signaling pathway
D0090023biological_processpositive regulation of neutrophil chemotaxis
D0097178biological_processruffle assembly
D0098794cellular_componentpostsynapse
D0098978cellular_componentglutamatergic synapse
D0101003cellular_componentficolin-1-rich granule membrane
D1900026biological_processpositive regulation of substrate adhesion-dependent cell spreading
D1902622biological_processregulation of neutrophil migration
E0005085molecular_functionguanyl-nucleotide exchange factor activity
E0035556biological_processintracellular signal transduction
F0001764biological_processneuron migration
F0001934biological_processpositive regulation of protein phosphorylation
F0003376biological_processsphingosine-1-phosphate receptor signaling pathway
F0003924molecular_functionGTPase activity
F0003925molecular_functionG protein activity
F0005515molecular_functionprotein binding
F0005525molecular_functionGTP binding
F0005634cellular_componentnucleus
F0005737cellular_componentcytoplasm
F0005789cellular_componentendoplasmic reticulum membrane
F0005802cellular_componenttrans-Golgi network
F0005829cellular_componentcytosol
F0005856cellular_componentcytoskeleton
F0005884cellular_componentactin filament
F0005886cellular_componentplasma membrane
F0005925cellular_componentfocal adhesion
F0005938cellular_componentcell cortex
F0006954biological_processinflammatory response
F0007015biological_processactin filament organization
F0007155biological_processcell adhesion
F0007160biological_processcell-matrix adhesion
F0007163biological_processestablishment or maintenance of cell polarity
F0007264biological_processsmall GTPase-mediated signal transduction
F0008045biological_processmotor neuron axon guidance
F0008360biological_processregulation of cell shape
F0008361biological_processregulation of cell size
F0009611biological_processresponse to wounding
F0009653biological_processanatomical structure morphogenesis
F0010310biological_processregulation of hydrogen peroxide metabolic process
F0010591biological_processregulation of lamellipodium assembly
F0010592biological_processpositive regulation of lamellipodium assembly
F0010595biological_processpositive regulation of endothelial cell migration
F0010764biological_processnegative regulation of fibroblast migration
F0010811biological_processpositive regulation of cell-substrate adhesion
F0016020cellular_componentmembrane
F0016477biological_processcell migration
F0016601biological_processRac protein signal transduction
F0016787molecular_functionhydrolase activity
F0019899molecular_functionenzyme binding
F0019901molecular_functionprotein kinase binding
F0030027cellular_componentlamellipodium
F0030031biological_processcell projection assembly
F0030032biological_processlamellipodium assembly
F0030036biological_processactin cytoskeleton organization
F0030041biological_processactin filament polymerization
F0030334biological_processregulation of cell migration
F0030425cellular_componentdendrite
F0030667cellular_componentsecretory granule membrane
F0030865biological_processcortical cytoskeleton organization
F0031116biological_processpositive regulation of microtubule polymerization
F0031410cellular_componentcytoplasmic vesicle
F0031529biological_processruffle organization
F0031996molecular_functionthioesterase binding
F0032587cellular_componentruffle membrane
F0032707biological_processnegative regulation of interleukin-23 production
F0032956biological_processregulation of actin cytoskeleton organization
F0034446biological_processsubstrate adhesion-dependent cell spreading
F0035025biological_processpositive regulation of Rho protein signal transduction
F0035556biological_processintracellular signal transduction
F0036464cellular_componentcytoplasmic ribonucleoprotein granule
F0042470cellular_componentmelanosome
F0042995cellular_componentcell projection
F0043020cellular_componentNADPH oxidase complex
F0043197cellular_componentdendritic spine
F0043652biological_processengulfment of apoptotic cell
F0044877molecular_functionprotein-containing complex binding
F0045202cellular_componentsynapse
F0045428biological_processregulation of nitric oxide biosynthetic process
F0045730biological_processrespiratory burst
F0048012biological_processhepatocyte growth factor receptor signaling pathway
F0048261biological_processnegative regulation of receptor-mediated endocytosis
F0048870biological_processcell motility
F0051022molecular_functionRho GDP-dissociation inhibitor binding
F0051492biological_processregulation of stress fiber assembly
F0051496biological_processpositive regulation of stress fiber assembly
F0051668biological_processlocalization within membrane
F0051894biological_processpositive regulation of focal adhesion assembly
F0055038cellular_componentrecycling endosome membrane
F0060071biological_processWnt signaling pathway, planar cell polarity pathway
F0060263biological_processregulation of respiratory burst
F0060326biological_processcell chemotaxis
F0070062cellular_componentextracellular exosome
F0071526biological_processsemaphorin-plexin signaling pathway
F0090023biological_processpositive regulation of neutrophil chemotaxis
F0097178biological_processruffle assembly
F0098794cellular_componentpostsynapse
F0098978cellular_componentglutamatergic synapse
F0101003cellular_componentficolin-1-rich granule membrane
F1900026biological_processpositive regulation of substrate adhesion-dependent cell spreading
F1902622biological_processregulation of neutrophil migration
G0005085molecular_functionguanyl-nucleotide exchange factor activity
G0035556biological_processintracellular signal transduction
H0001764biological_processneuron migration
H0001934biological_processpositive regulation of protein phosphorylation
H0003376biological_processsphingosine-1-phosphate receptor signaling pathway
H0003924molecular_functionGTPase activity
H0003925molecular_functionG protein activity
H0005515molecular_functionprotein binding
H0005525molecular_functionGTP binding
H0005634cellular_componentnucleus
H0005737cellular_componentcytoplasm
H0005789cellular_componentendoplasmic reticulum membrane
H0005802cellular_componenttrans-Golgi network
H0005829cellular_componentcytosol
H0005856cellular_componentcytoskeleton
H0005884cellular_componentactin filament
H0005886cellular_componentplasma membrane
H0005925cellular_componentfocal adhesion
H0005938cellular_componentcell cortex
H0006954biological_processinflammatory response
H0007015biological_processactin filament organization
H0007155biological_processcell adhesion
H0007160biological_processcell-matrix adhesion
H0007163biological_processestablishment or maintenance of cell polarity
H0007264biological_processsmall GTPase-mediated signal transduction
H0008045biological_processmotor neuron axon guidance
H0008360biological_processregulation of cell shape
H0008361biological_processregulation of cell size
H0009611biological_processresponse to wounding
H0009653biological_processanatomical structure morphogenesis
H0010310biological_processregulation of hydrogen peroxide metabolic process
H0010591biological_processregulation of lamellipodium assembly
H0010592biological_processpositive regulation of lamellipodium assembly
H0010595biological_processpositive regulation of endothelial cell migration
H0010764biological_processnegative regulation of fibroblast migration
H0010811biological_processpositive regulation of cell-substrate adhesion
H0016020cellular_componentmembrane
H0016477biological_processcell migration
H0016601biological_processRac protein signal transduction
H0016787molecular_functionhydrolase activity
H0019899molecular_functionenzyme binding
H0019901molecular_functionprotein kinase binding
H0030027cellular_componentlamellipodium
H0030031biological_processcell projection assembly
H0030032biological_processlamellipodium assembly
H0030036biological_processactin cytoskeleton organization
H0030041biological_processactin filament polymerization
H0030334biological_processregulation of cell migration
H0030425cellular_componentdendrite
H0030667cellular_componentsecretory granule membrane
H0030865biological_processcortical cytoskeleton organization
H0031116biological_processpositive regulation of microtubule polymerization
H0031410cellular_componentcytoplasmic vesicle
H0031529biological_processruffle organization
H0031996molecular_functionthioesterase binding
H0032587cellular_componentruffle membrane
H0032707biological_processnegative regulation of interleukin-23 production
H0032956biological_processregulation of actin cytoskeleton organization
H0034446biological_processsubstrate adhesion-dependent cell spreading
H0035025biological_processpositive regulation of Rho protein signal transduction
H0035556biological_processintracellular signal transduction
H0036464cellular_componentcytoplasmic ribonucleoprotein granule
H0042470cellular_componentmelanosome
H0042995cellular_componentcell projection
H0043020cellular_componentNADPH oxidase complex
H0043197cellular_componentdendritic spine
H0043652biological_processengulfment of apoptotic cell
H0044877molecular_functionprotein-containing complex binding
H0045202cellular_componentsynapse
H0045428biological_processregulation of nitric oxide biosynthetic process
H0045730biological_processrespiratory burst
H0048012biological_processhepatocyte growth factor receptor signaling pathway
H0048261biological_processnegative regulation of receptor-mediated endocytosis
H0048870biological_processcell motility
H0051022molecular_functionRho GDP-dissociation inhibitor binding
H0051492biological_processregulation of stress fiber assembly
H0051496biological_processpositive regulation of stress fiber assembly
H0051668biological_processlocalization within membrane
H0051894biological_processpositive regulation of focal adhesion assembly
H0055038cellular_componentrecycling endosome membrane
H0060071biological_processWnt signaling pathway, planar cell polarity pathway
H0060263biological_processregulation of respiratory burst
H0060326biological_processcell chemotaxis
H0070062cellular_componentextracellular exosome
H0071526biological_processsemaphorin-plexin signaling pathway
H0090023biological_processpositive regulation of neutrophil chemotaxis
H0097178biological_processruffle assembly
H0098794cellular_componentpostsynapse
H0098978cellular_componentglutamatergic synapse
H0101003cellular_componentficolin-1-rich granule membrane
H1900026biological_processpositive regulation of substrate adhesion-dependent cell spreading
H1902622biological_processregulation of neutrophil migration
Functional Information from PDB Data
site_idAC1
Number of Residues3
Detailsbinding site for residue PO4 A 501
ChainResidue
AARG45
ALYS211
AARG212

site_idAC2
Number of Residues3
Detailsbinding site for residue PO4 C 501
ChainResidue
CARG45
CLYS211
CARG212

site_idAC3
Number of Residues3
Detailsbinding site for residue PO4 E 501
ChainResidue
EARG45
ELYS211
EARG212

site_idAC4
Number of Residues3
Detailsbinding site for residue PO4 G 501
ChainResidue
GARG45
GLYS211
GARG212

Functional Information from PROSITE/UniProt
site_idPS00741
Number of Residues26
DetailsDH_1 Dbl homology (DH) domain signature. LegYLlsPIqrIcKypLlLkELakrT
ChainResidueDetails
ALEU188-THR213

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues12
DetailsBINDING: BINDING => ECO:0000269|PubMed:11090627, ECO:0007744|PDB:1E96, ECO:0007744|PDB:2WKP, ECO:0007744|PDB:2WKQ, ECO:0007744|PDB:2WKR
ChainResidueDetails
FGLY60
HALA13
HGLY30
HGLY60
BALA13
BGLY30
BGLY60
DALA13
DGLY30
DGLY60
FALA13
FGLY30

site_idSWS_FT_FI2
Number of Residues8
DetailsBINDING: BINDING => ECO:0000269|PubMed:11090627, ECO:0007744|PDB:1E96, ECO:0007744|PDB:2WKP, ECO:0007744|PDB:2WKQ, ECO:0007744|PDB:2WKR, ECO:0007744|PDB:5HZH
ChainResidueDetails
BLYS116
BALA159
FLYS116
FALA159
HLYS116
HALA159
DLYS116
DALA159

site_idSWS_FT_FI3
Number of Residues4
DetailsMOD_RES: (Microbial infection) O-AMP-tyrosine; by Haemophilus IbpA; alternate => ECO:0000269|PubMed:19362538
ChainResidueDetails
BTYR32
DTYR32
FTYR32
HTYR32

site_idSWS_FT_FI4
Number of Residues4
DetailsMOD_RES: (Microbial infection) O-AMP-threonine; by Vibrio VopS => ECO:0000269|PubMed:19039103
ChainResidueDetails
BTHR35
HTHR35
DTHR35
FTHR35

site_idSWS_FT_FI5
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0000269|PubMed:10617634
ChainResidueDetails
BSER71
HSER71
DSER71
FSER71

site_idSWS_FT_FI6
Number of Residues4
DetailsMOD_RES: Cysteine methyl ester => ECO:0000250|UniProtKB:P61585
ChainResidueDetails
BCYS189
HCYS189
DCYS189
FCYS189

site_idSWS_FT_FI7
Number of Residues8
DetailsLIPID: (Microbial infection) N6-palmitoyl lysine => ECO:0000269|PubMed:29074776
ChainResidueDetails
FLYS183
FLYS184
HLYS183
HLYS184
BLYS183
BLYS184
DLYS183
DLYS184

site_idSWS_FT_FI8
Number of Residues4
DetailsLIPID: S-geranylgeranyl cysteine => ECO:0000269|PubMed:1903399
ChainResidueDetails
HCYS189
BCYS189
DCYS189
FCYS189

site_idSWS_FT_FI9
Number of Residues4
DetailsCARBOHYD: (Microbial infection) O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230; alternate => ECO:0000269|PubMed:24141704
ChainResidueDetails
HTYR32
BTYR32
DTYR32
FTYR32

site_idSWS_FT_FI10
Number of Residues4
DetailsCARBOHYD: (Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB, and by P.sordellii toxin TcsL; alternate => ECO:0000269|PubMed:19744486, ECO:0000269|PubMed:24905543, ECO:0000269|PubMed:7775453, ECO:0000269|PubMed:7777059
ChainResidueDetails
DTHR35
HTHR35
BTHR35
FTHR35

site_idSWS_FT_FI11
Number of Residues4
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:18093184
ChainResidueDetails
BLYS147
DLYS147
FLYS147
HLYS147

site_idSWS_FT_FI12
Number of Residues8
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:23512198
ChainResidueDetails
BLYS166
DLYS166
FLYS166
HLYS166

219140

PDB entries from 2024-05-01

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