5FI0

Crystal Structure of the P-Rex1 DH/PH tandem in complex with Rac1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	110
Detector technology	CCD
Collection date	2013-07-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.127
Spacegroup name	P 21 21 21
Unit cell lengths	81.373, 107.053, 323.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.847 - 3.282
R-factor	0.1924
Rwork	0.190
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2DFK and 5D3W
RMSD bond length	0.005
RMSD bond angle	1.048
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.310
High resolution limit [Å]	3.250	8.780	3.250
Rmerge	0.173	0.066	0.870
Rmeas	0.190	0.072	0.952
Rpim	0.076	0.029	0.377
Total number of observations	247146
Number of reflections	42862
<I/σ(I)>	5.6
Completeness [%]	98.0	98.7	91.9
Redundancy	5.8	6	5.5
CC(1/2)		0.996	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	Sodium dihydrogen phosphate, PEG 8000