5E8J
Crystal structure of mRNA cap guanine-N7 methyltransferase in complex with RAM
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| A | 0006370 | biological_process | 7-methylguanosine mRNA capping |
| B | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| B | 0006370 | biological_process | 7-methylguanosine mRNA capping |
| C | 0003723 | molecular_function | RNA binding |
| C | 0031533 | cellular_component | mRNA capping enzyme complex |
| C | 0106005 | biological_process | RNA 5'-cap (guanine-N7)-methylation |
| D | 0003723 | molecular_function | RNA binding |
| D | 0031533 | cellular_component | mRNA capping enzyme complex |
| D | 0106005 | biological_process | RNA 5'-cap (guanine-N7)-methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 501 |
| Chain | Residue |
| A | GLY207 |
| A | LYS208 |
| A | GLY209 |
| A | GLY210 |
| A | SAH502 |
| A | HOH633 |
| site_id | AC2 |
| Number of Residues | 18 |
| Details | binding site for residue SAH A 502 |
| Chain | Residue |
| A | GLY207 |
| A | ASP227 |
| A | ILE228 |
| A | ALA260 |
| A | ASP261 |
| A | SER262 |
| A | GLN284 |
| A | PHE285 |
| A | VAL286 |
| A | TYR289 |
| A | MET300 |
| A | GOL501 |
| A | HOH633 |
| A | HOH654 |
| A | HOH663 |
| A | LYS180 |
| A | GLY205 |
| A | CYS206 |
| site_id | AC3 |
| Number of Residues | 14 |
| Details | binding site for residue SAH B 501 |
| Chain | Residue |
| B | LYS180 |
| B | GLY205 |
| B | CYS206 |
| B | GLY207 |
| B | ASP227 |
| B | ILE228 |
| B | ALA260 |
| B | ASP261 |
| B | SER262 |
| B | GLN284 |
| B | PHE285 |
| B | VAL286 |
| B | TYR289 |
| B | HOH650 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | MOD_RES: N-acetylthreonine => ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| C | THR2 | |
| D | THR2 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18220336, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| C | SER36 | |
| D | SER36 | |
| A | ASP227 | |
| B | LYS180 | |
| B | GLY205 | |
| B | ASP227 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27422871, ECO:0007744|PDB:3BGV, ECO:0007744|PDB:5E9J, ECO:0007744|PDB:5E9W |
| Chain | Residue | Details |
| A | ASP261 | |
| A | GLN284 | |
| A | TYR289 | |
| B | ASP261 | |
| B | GLN284 | |
| B | TYR289 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00895 |
| Chain | Residue | Details |
| A | LYS208 | |
| B | HIS288 | |
| B | GLU370 | |
| B | TYR467 | |
| A | LYS214 | |
| A | ARG239 | |
| A | HIS288 | |
| A | GLU370 | |
| A | TYR467 | |
| B | LYS208 | |
| B | LYS214 | |
| B | ARG239 |
