4KBL
Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000151 | cellular_component | ubiquitin ligase complex |
| A | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0015030 | cellular_component | Cajal body |
| A | 0016567 | biological_process | protein ubiquitination |
| A | 0016604 | cellular_component | nuclear body |
| A | 0016740 | molecular_function | transferase activity |
| A | 0019005 | cellular_component | SCF ubiquitin ligase complex |
| A | 0019787 | molecular_function | ubiquitin-like protein transferase activity |
| A | 0031462 | cellular_component | Cul2-RING ubiquitin ligase complex |
| A | 0031463 | cellular_component | Cul3-RING ubiquitin ligase complex |
| A | 0031464 | cellular_component | Cul4A-RING E3 ubiquitin ligase complex |
| A | 0031624 | molecular_function | ubiquitin conjugating enzyme binding |
| A | 0031625 | molecular_function | ubiquitin protein ligase binding |
| A | 0039585 | biological_process | PKR/eIFalpha signaling |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0061630 | molecular_function | ubiquitin protein ligase activity |
| A | 0097413 | cellular_component | Lewy body |
| B | 0000151 | cellular_component | ubiquitin ligase complex |
| B | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0015030 | cellular_component | Cajal body |
| B | 0016567 | biological_process | protein ubiquitination |
| B | 0016604 | cellular_component | nuclear body |
| B | 0016740 | molecular_function | transferase activity |
| B | 0019005 | cellular_component | SCF ubiquitin ligase complex |
| B | 0019787 | molecular_function | ubiquitin-like protein transferase activity |
| B | 0031462 | cellular_component | Cul2-RING ubiquitin ligase complex |
| B | 0031463 | cellular_component | Cul3-RING ubiquitin ligase complex |
| B | 0031464 | cellular_component | Cul4A-RING E3 ubiquitin ligase complex |
| B | 0031624 | molecular_function | ubiquitin conjugating enzyme binding |
| B | 0031625 | molecular_function | ubiquitin protein ligase binding |
| B | 0039585 | biological_process | PKR/eIFalpha signaling |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0061630 | molecular_function | ubiquitin protein ligase activity |
| B | 0097413 | cellular_component | Lewy body |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 601 |
| Chain | Residue |
| A | CYS344 |
| A | CYS347 |
| A | CYS362 |
| A | CYS367 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 602 |
| Chain | Residue |
| A | CYS372 |
| A | CYS375 |
| A | HIS382 |
| A | CYS389 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 603 |
| Chain | Residue |
| A | CYS281 |
| A | CYS297 |
| A | CYS299 |
| A | CYS276 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 604 |
| Chain | Residue |
| A | CYS304 |
| A | CYS307 |
| A | HIS312 |
| A | CYS317 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 605 |
| Chain | Residue |
| A | CYS186 |
| A | CYS189 |
| A | CYS208 |
| A | CYS211 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 606 |
| Chain | Residue |
| A | CYS203 |
| A | HIS205 |
| A | CYS231 |
| A | CYS236 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 601 |
| Chain | Residue |
| B | CYS344 |
| B | CYS347 |
| B | CYS362 |
| B | CYS367 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 602 |
| Chain | Residue |
| B | CYS372 |
| B | CYS375 |
| B | HIS382 |
| B | CYS389 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 603 |
| Chain | Residue |
| B | CYS276 |
| B | CYS281 |
| B | CYS297 |
| B | CYS299 |
| site_id | BC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 604 |
| Chain | Residue |
| B | CYS304 |
| B | CYS307 |
| B | HIS312 |
| B | CYS317 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 605 |
| Chain | Residue |
| B | CYS186 |
| B | CYS189 |
| B | CYS208 |
| B | CYS211 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 606 |
| Chain | Residue |
| B | CYS203 |
| B | HIS205 |
| B | CYS231 |
| B | CYS236 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 50 |
| Details | Zinc finger: {"description":"RING-type 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU01221","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 122 |
| Details | Zinc finger: {"description":"IBR-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU01221","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 62 |
| Details | Zinc finger: {"description":"RING-type 2; atypical","evidences":[{"source":"PROSITE-ProRule","id":"PRU01221","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 48 |
| Details | Region: {"description":"UBA-like","evidences":[{"source":"PubMed","id":"23707686","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01221","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"21532592","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23707686","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 32 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01221","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23707686","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4KBL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KC9","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01221","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15236971","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23707686","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24058416","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1WD2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2M9Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KBL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KC9","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01221","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23707686","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24058416","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2M9Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KBL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4KC9","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q9Z1K5","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
