4KBL

Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 41
Unit cell lengths	147.403, 147.403, 86.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.613 - 3.300
R-factor	0.2353
Rwork	0.231
R-free	0.28310
RMSD bond length	0.008
RMSD bond angle	1.393
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX ((Autosol))
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.670	3.480
High resolution limit [Å]	3.300	3.300
Completeness [%]	98.1	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	0.92M potassium / sodium tartrate, 0.2M lithium sulfate, 0.1M CHES, 5% ethylene glycol, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K