3Q7E

Crystal Structure of rat Protein Arginine Methyltransferase 1 (PRMT1)M48L mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000724	biological_process	double-strand break repair via homologous recombination
A	0001701	biological_process	in utero embryonic development
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005829	cellular_component	cytosol
A	0006338	biological_process	chromatin remodeling
A	0006355	biological_process	regulation of DNA-templated transcription
A	0006479	biological_process	protein methylation
A	0006974	biological_process	DNA damage response
A	0008168	molecular_function	methyltransferase activity
A	0008170	molecular_function	N-methyltransferase activity
A	0008276	molecular_function	protein methyltransferase activity
A	0008284	biological_process	positive regulation of cell population proliferation
A	0008327	molecular_function	methyl-CpG binding
A	0008380	biological_process	RNA splicing
A	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
A	0016274	molecular_function	protein-arginine N-methyltransferase activity
A	0016740	molecular_function	transferase activity
A	0017148	biological_process	negative regulation of translation
A	0018216	biological_process	peptidyl-arginine methylation
A	0019899	molecular_function	enzyme binding
A	0019919	biological_process	peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
A	0030509	biological_process	BMP signaling pathway
A	0030510	biological_process	regulation of BMP signaling pathway
A	0030514	biological_process	negative regulation of BMP signaling pathway
A	0030519	molecular_function	snoRNP binding
A	0031175	biological_process	neuron projection development
A	0031669	biological_process	cellular response to nutrient levels
A	0032259	biological_process	methylation
A	0032991	cellular_component	protein-containing complex
A	0034198	biological_process	cellular response to amino acid starvation
A	0034709	cellular_component	methylosome
A	0035241	molecular_function	protein-arginine omega-N monomethyltransferase activity
A	0035242	molecular_function	protein-arginine omega-N asymmetric methyltransferase activity
A	0035247	biological_process	peptidyl-arginine omega-N-methylation
A	0038202	biological_process	TORC1 signaling
A	0042054	molecular_function	histone methyltransferase activity
A	0042802	molecular_function	identical protein binding
A	0044020	molecular_function	histone H4R3 methyltransferase activity
A	0045648	biological_process	positive regulation of erythrocyte differentiation
A	0045652	biological_process	regulation of megakaryocyte differentiation
A	0045653	biological_process	negative regulation of megakaryocyte differentiation
A	0045727	biological_process	positive regulation of translation
A	0045947	biological_process	negative regulation of translational initiation
A	0046329	biological_process	negative regulation of JNK cascade
A	0046500	biological_process	S-adenosylmethionine metabolic process
A	0046985	biological_process	positive regulation of hemoglobin biosynthetic process
A	0048273	molecular_function	mitogen-activated protein kinase p38 binding
A	0048738	biological_process	cardiac muscle tissue development
A	0051260	biological_process	protein homooligomerization
A	0061431	biological_process	cellular response to methionine
A	0061462	biological_process	protein localization to lysosome
A	0097421	biological_process	liver regeneration
A	0106080	molecular_function	GATOR1 complex binding
A	0140694	biological_process	membraneless organelle assembly
A	1900745	biological_process	positive regulation of p38MAPK cascade
A	1904047	molecular_function	S-adenosyl-L-methionine binding
A	1904262	biological_process	negative regulation of TORC1 signaling
A	1904263	biological_process	positive regulation of TORC1 signaling
A	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
A	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE SAH A 1

Chain	Residue
A	HIS45
A	GLY126
A	LYS127
A	VAL128
A	GLU129
A	GLU144
A	MET155
A	HOH355
A	HOH363
A	HOH364
A	LEU48
A	ARG54
A	GLY78
A	SER79
A	ILE83
A	LEU84
A	GLU100
A	CYS101

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 354

Chain	Residue
A	ARG59
A	ASN60
A	PHE63

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"evidences":[{"source":"PubMed","id":"12737817","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12737817","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q9JIF0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q9JIF0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q99873","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI6
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"Q9JIF0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 872

Chain	Residue	Details
A	ASP51	proton shuttle (general acid/base)
A	GLU144	electrostatic stabiliser
A	GLU153	electrostatic stabiliser
A	HIS293	proton shuttle (general acid/base)

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