3Q7E

Crystal Structure of rat Protein Arginine Methyltransferase 1 (PRMT1)M48L mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	85
Detector technology	IMAGE PLATE
Collection date	2009-08-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 2 2
Unit cell lengths	87.307, 87.307, 143.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.692 - 2.200
R-factor	0.2033
Rwork	0.201
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1or8
RMSD bond length	0.008
RMSD bond angle	1.124
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER (1.3.2)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.094	0.437
Number of reflections	27377
<I/σ(I)>	8.9	2.4
Completeness [%]	94.8	68
Redundancy	4.78	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	295	0.1 M Tris, 1.62 M ammonium phosphate monobasic, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K