3PFW

Crystal structure of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) complex with NAD, a binary form

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0006006	biological_process	glucose metabolic process
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0006006	biological_process	glucose metabolic process
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	32
Details	BINDING SITE FOR RESIDUE NAD O 2

Chain	Residue
O	HOH27
O	GLY84
O	ARG85
O	ILE86
O	ASP106
O	PRO107
O	PHE108
O	ILE109
O	LYS151
O	SER169
O	THR170
O	HOH30
O	GLY171
O	TYR173
O	SER193
O	ALA194
O	ALA255
O	ASN388
O	TYR392
O	GOL416
O	HOH423
O	HOH444
O	HOH31
O	HOH457
O	HOH459
O	HOH491
O	HOH38
O	HOH47
O	HOH55
O	ASN81
O	GLY82
O	PHE83

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL O 1

Chain	Residue
O	GLY205
O	VAL206
O	GLU208
O	ASN209
O	LYS234

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL O 415

Chain	Residue
O	SER223
O	THR225
O	HIS251
O	THR283
O	ALA304
O	ARG306
O	HOH499

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL O 416

Chain	Residue
O	NAD2
O	THR254
O	THR256
O	ARG306
O	HOH459

---

site_id	AC5
Number of Residues	30
Details	BINDING SITE FOR RESIDUE NAD P 1

Chain	Residue
P	ASN81
P	GLY82
P	PHE83
P	GLY84
P	ARG85
P	ILE86
P	ASP106
P	PRO107
P	PHE108
P	LYS151
P	SER169
P	THR170
P	GLY171
P	TYR173
P	SER193
P	ALA194
P	CYS224
P	ALA255
P	PRO263
P	ASN388
P	TYR392
P	GOL416
P	HOH440
P	HOH448
P	HOH451
P	HOH474
P	HOH489
P	HOH517
P	HOH525
P	HOH555

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL P 415

Chain	Residue
P	SER223
P	CYS224
P	THR225
P	HIS251
P	THR283
P	ALA304
P	ARG306
P	HOH492

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL P 416

Chain	Residue
P	NAD1
P	THR254
P	THR256
P	ARG306
P	HOH451
P	HOH517
P	HOH531

---

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA222-LEU229

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile => ECO:0000255|PROSITE-ProRule:PRU10009, ECO:0000269|PubMed:21269272

Chain	Residue	Details
O	CYS224
P	CYS224

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:21269272

Chain	Residue	Details
O	ARG85
P	TYR173
P	SER193
P	ASN388
O	ASP106
O	LYS151
O	TYR173
O	SER193
O	ASN388
P	ARG85
P	ASP106
P	LYS151

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
O	SER223
O	THR254
O	THR283
O	ARG306
P	SER223
P	THR254
P	THR283
P	ARG306

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Activates thiol group during catalysis

Chain	Residue	Details
O	HIS251
P	HIS251

---