3PFW
Crystal structure of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) complex with NAD, a binary form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-12 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.610, 71.860, 81.100 |
| Unit cell angles | 90.00, 123.32, 90.00 |
Refinement procedure
| Resolution | 67.770 - 2.150 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h9e |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.540 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.770 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.171 | 0.671 |
| Number of reflections | 35287 | |
| <I/σ(I)> | 7 | 2.3 |
| Completeness [%] | 94.1 | 92 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 277.15 | 20% PEG 3350, 0.2 M Na2SO4, 10% ethylene glycol and 0.1 M Bis-Tris propane pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
