2V64

Crystallographic structure of the conformational dimer of the Spindle Assembly Checkpoint protein Mad2.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000070	biological_process	mitotic sister chromatid segregation
A	0000132	biological_process	establishment of mitotic spindle orientation
A	0000775	cellular_component	chromosome, centromeric region
A	0000776	cellular_component	kinetochore
A	0000922	cellular_component	spindle pole
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005694	cellular_component	chromosome
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0007094	biological_process	mitotic spindle assembly checkpoint signaling
A	0033597	cellular_component	mitotic checkpoint complex
A	0042177	biological_process	negative regulation of protein catabolic process
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0043066	biological_process	negative regulation of apoptotic process
A	0044615	cellular_component	nuclear pore nuclear basket
A	0045930	biological_process	negative regulation of mitotic cell cycle
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0051301	biological_process	cell division
A	0051660	biological_process	establishment of centrosome localization
A	0072686	cellular_component	mitotic spindle
A	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
A	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
A	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
C	0000070	biological_process	mitotic sister chromatid segregation
C	0000132	biological_process	establishment of mitotic spindle orientation
C	0000775	cellular_component	chromosome, centromeric region
C	0000776	cellular_component	kinetochore
C	0000922	cellular_component	spindle pole
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005694	cellular_component	chromosome
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0007094	biological_process	mitotic spindle assembly checkpoint signaling
C	0033597	cellular_component	mitotic checkpoint complex
C	0042177	biological_process	negative regulation of protein catabolic process
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0043066	biological_process	negative regulation of apoptotic process
C	0044615	cellular_component	nuclear pore nuclear basket
C	0045930	biological_process	negative regulation of mitotic cell cycle
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0051301	biological_process	cell division
C	0051660	biological_process	establishment of centrosome localization
C	0072686	cellular_component	mitotic spindle
C	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
C	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
C	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
D	0000070	biological_process	mitotic sister chromatid segregation
D	0000132	biological_process	establishment of mitotic spindle orientation
D	0000775	cellular_component	chromosome, centromeric region
D	0000776	cellular_component	kinetochore
D	0000922	cellular_component	spindle pole
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0007094	biological_process	mitotic spindle assembly checkpoint signaling
D	0033597	cellular_component	mitotic checkpoint complex
D	0042177	biological_process	negative regulation of protein catabolic process
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0043066	biological_process	negative regulation of apoptotic process
D	0044615	cellular_component	nuclear pore nuclear basket
D	0045930	biological_process	negative regulation of mitotic cell cycle
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0051301	biological_process	cell division
D	0051660	biological_process	establishment of centrosome localization
D	0072686	cellular_component	mitotic spindle
D	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
D	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
D	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
E	0000070	biological_process	mitotic sister chromatid segregation
E	0000132	biological_process	establishment of mitotic spindle orientation
E	0000775	cellular_component	chromosome, centromeric region
E	0000776	cellular_component	kinetochore
E	0000922	cellular_component	spindle pole
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005694	cellular_component	chromosome
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0007094	biological_process	mitotic spindle assembly checkpoint signaling
E	0033597	cellular_component	mitotic checkpoint complex
E	0042177	biological_process	negative regulation of protein catabolic process
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0043066	biological_process	negative regulation of apoptotic process
E	0044615	cellular_component	nuclear pore nuclear basket
E	0045930	biological_process	negative regulation of mitotic cell cycle
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0051301	biological_process	cell division
E	0051660	biological_process	establishment of centrosome localization
E	0072686	cellular_component	mitotic spindle
E	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
E	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
E	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
F	0000070	biological_process	mitotic sister chromatid segregation
F	0000132	biological_process	establishment of mitotic spindle orientation
F	0000775	cellular_component	chromosome, centromeric region
F	0000776	cellular_component	kinetochore
F	0000922	cellular_component	spindle pole
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005694	cellular_component	chromosome
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0007094	biological_process	mitotic spindle assembly checkpoint signaling
F	0033597	cellular_component	mitotic checkpoint complex
F	0042177	biological_process	negative regulation of protein catabolic process
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0043066	biological_process	negative regulation of apoptotic process
F	0044615	cellular_component	nuclear pore nuclear basket
F	0045930	biological_process	negative regulation of mitotic cell cycle
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0051301	biological_process	cell division
F	0051660	biological_process	establishment of centrosome localization
F	0072686	cellular_component	mitotic spindle
F	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
F	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
F	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
H	0000070	biological_process	mitotic sister chromatid segregation
H	0000132	biological_process	establishment of mitotic spindle orientation
H	0000775	cellular_component	chromosome, centromeric region
H	0000776	cellular_component	kinetochore
H	0000922	cellular_component	spindle pole
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005694	cellular_component	chromosome
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005856	cellular_component	cytoskeleton
H	0007094	biological_process	mitotic spindle assembly checkpoint signaling
H	0033597	cellular_component	mitotic checkpoint complex
H	0042177	biological_process	negative regulation of protein catabolic process
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0043066	biological_process	negative regulation of apoptotic process
H	0044615	cellular_component	nuclear pore nuclear basket
H	0045930	biological_process	negative regulation of mitotic cell cycle
H	0048471	cellular_component	perinuclear region of cytoplasm
H	0051301	biological_process	cell division
H	0051660	biological_process	establishment of centrosome localization
H	0072686	cellular_component	mitotic spindle
H	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
H	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
H	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	MOD_RES: N-acetylalanine => ECO:0000269|Ref.12

Chain	Residue	Details
D	ALA2
E	ALA2
H	ALA2

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site_id	SWS_FT_FI2
Number of Residues	9
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
D	SER6
D	SER130
D	SER185
E	SER6
E	SER130
E	SER185
H	SER6
H	SER130
H	SER185

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:12574116

Chain	Residue	Details
D	SER170
D	SER178
E	SER170
E	SER178
H	SER170
H	SER178

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:12574116, ECO:0007744|PubMed:23186163

Chain	Residue	Details
D	SER195
E	SER195
H	SER195

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