Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2V64

Crystallographic structure of the conformational dimer of the Spindle Assembly Checkpoint protein Mad2.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000775	cellular_component	chromosome, centromeric region
A	0000776	cellular_component	kinetochore
A	0000922	cellular_component	spindle pole
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005694	cellular_component	chromosome
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0007094	biological_process	mitotic spindle assembly checkpoint signaling
A	0042177	biological_process	negative regulation of protein catabolic process
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0044615	cellular_component	nuclear pore nuclear basket
A	0045930	biological_process	negative regulation of mitotic cell cycle
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0051301	biological_process	cell division
A	0051783	biological_process	regulation of nuclear division
A	0072686	cellular_component	mitotic spindle
A	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
A	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
A	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
A	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
C	0000775	cellular_component	chromosome, centromeric region
C	0000776	cellular_component	kinetochore
C	0000922	cellular_component	spindle pole
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005694	cellular_component	chromosome
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0007094	biological_process	mitotic spindle assembly checkpoint signaling
C	0042177	biological_process	negative regulation of protein catabolic process
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0044615	cellular_component	nuclear pore nuclear basket
C	0045930	biological_process	negative regulation of mitotic cell cycle
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0051301	biological_process	cell division
C	0051783	biological_process	regulation of nuclear division
C	0072686	cellular_component	mitotic spindle
C	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
C	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
C	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
C	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
D	0000775	cellular_component	chromosome, centromeric region
D	0000776	cellular_component	kinetochore
D	0000922	cellular_component	spindle pole
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0007094	biological_process	mitotic spindle assembly checkpoint signaling
D	0042177	biological_process	negative regulation of protein catabolic process
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0044615	cellular_component	nuclear pore nuclear basket
D	0045930	biological_process	negative regulation of mitotic cell cycle
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0051301	biological_process	cell division
D	0051783	biological_process	regulation of nuclear division
D	0072686	cellular_component	mitotic spindle
D	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
D	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
D	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
D	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
E	0000775	cellular_component	chromosome, centromeric region
E	0000776	cellular_component	kinetochore
E	0000922	cellular_component	spindle pole
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005694	cellular_component	chromosome
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0007094	biological_process	mitotic spindle assembly checkpoint signaling
E	0042177	biological_process	negative regulation of protein catabolic process
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0044615	cellular_component	nuclear pore nuclear basket
E	0045930	biological_process	negative regulation of mitotic cell cycle
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0051301	biological_process	cell division
E	0051783	biological_process	regulation of nuclear division
E	0072686	cellular_component	mitotic spindle
E	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
E	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
E	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
E	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
F	0000775	cellular_component	chromosome, centromeric region
F	0000776	cellular_component	kinetochore
F	0000922	cellular_component	spindle pole
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005694	cellular_component	chromosome
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0007094	biological_process	mitotic spindle assembly checkpoint signaling
F	0042177	biological_process	negative regulation of protein catabolic process
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0044615	cellular_component	nuclear pore nuclear basket
F	0045930	biological_process	negative regulation of mitotic cell cycle
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0051301	biological_process	cell division
F	0051783	biological_process	regulation of nuclear division
F	0072686	cellular_component	mitotic spindle
F	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
F	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
F	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
F	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
H	0000775	cellular_component	chromosome, centromeric region
H	0000776	cellular_component	kinetochore
H	0000922	cellular_component	spindle pole
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005694	cellular_component	chromosome
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0007094	biological_process	mitotic spindle assembly checkpoint signaling
H	0042177	biological_process	negative regulation of protein catabolic process
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0044615	cellular_component	nuclear pore nuclear basket
H	0045930	biological_process	negative regulation of mitotic cell cycle
H	0048471	cellular_component	perinuclear region of cytoplasm
H	0051301	biological_process	cell division
H	0051783	biological_process	regulation of nuclear division
H	0072686	cellular_component	mitotic spindle
H	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
H	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
H	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
H	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	549
Details	Domain: {"description":"HORMA","evidences":[{"source":"PROSITE-ProRule","id":"PRU00109","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	30
Details	Region: {"description":"Required for assuming the closed conformation and for interaction with CDC20"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	3
Details	Modified residue: {"description":"N-acetylalanine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Dhillon A.S.","Kolch W."]}}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	15
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	11
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"12574116","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"12574116","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)