2V64
Crystallographic structure of the conformational dimer of the Spindle Assembly Checkpoint protein Mad2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2006-10-10 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.580, 111.880, 131.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.440 - 2.900 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1go4 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.010 | 0.450 |
Number of reflections | 37591 | |
<I/σ(I)> | 16.2 | 4.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 0.1M NAACETATE PH 4.6, 3.5M NAFORMATE |