2V0T
The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004807 | molecular_function | triose-phosphate isomerase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006094 | biological_process | gluconeogenesis |
| A | 0006096 | biological_process | glycolytic process |
| A | 0016853 | molecular_function | isomerase activity |
| A | 0019563 | biological_process | glycerol catabolic process |
| A | 0020015 | cellular_component | glycosome |
| A | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| B | 0004807 | molecular_function | triose-phosphate isomerase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006094 | biological_process | gluconeogenesis |
| B | 0006096 | biological_process | glycolytic process |
| B | 0016853 | molecular_function | isomerase activity |
| B | 0019563 | biological_process | glycerol catabolic process |
| B | 0020015 | cellular_component | glycosome |
| B | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| C | 0004807 | molecular_function | triose-phosphate isomerase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006094 | biological_process | gluconeogenesis |
| C | 0006096 | biological_process | glycolytic process |
| C | 0016853 | molecular_function | isomerase activity |
| C | 0019563 | biological_process | glycerol catabolic process |
| C | 0020015 | cellular_component | glycosome |
| C | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| D | 0004807 | molecular_function | triose-phosphate isomerase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006094 | biological_process | gluconeogenesis |
| D | 0006096 | biological_process | glycolytic process |
| D | 0016853 | molecular_function | isomerase activity |
| D | 0019563 | biological_process | glycerol catabolic process |
| D | 0020015 | cellular_component | glycosome |
| D | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| E | 0004807 | molecular_function | triose-phosphate isomerase activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0006094 | biological_process | gluconeogenesis |
| E | 0006096 | biological_process | glycolytic process |
| E | 0016853 | molecular_function | isomerase activity |
| E | 0019563 | biological_process | glycerol catabolic process |
| E | 0020015 | cellular_component | glycosome |
| E | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| F | 0004807 | molecular_function | triose-phosphate isomerase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0006094 | biological_process | gluconeogenesis |
| F | 0006096 | biological_process | glycolytic process |
| F | 0016853 | molecular_function | isomerase activity |
| F | 0019563 | biological_process | glycerol catabolic process |
| F | 0020015 | cellular_component | glycosome |
| F | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| G | 0004807 | molecular_function | triose-phosphate isomerase activity |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005829 | cellular_component | cytosol |
| G | 0006094 | biological_process | gluconeogenesis |
| G | 0006096 | biological_process | glycolytic process |
| G | 0016853 | molecular_function | isomerase activity |
| G | 0019563 | biological_process | glycerol catabolic process |
| G | 0020015 | cellular_component | glycosome |
| G | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
| H | 0004807 | molecular_function | triose-phosphate isomerase activity |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0005829 | cellular_component | cytosol |
| H | 0006094 | biological_process | gluconeogenesis |
| H | 0006096 | biological_process | glycolytic process |
| H | 0016853 | molecular_function | isomerase activity |
| H | 0019563 | biological_process | glycerol catabolic process |
| H | 0020015 | cellular_component | glycosome |
| H | 0046166 | biological_process | glyceraldehyde-3-phosphate biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 A1251 |
| Chain | Residue |
| A | SER213 |
| A | GLY234 |
| A | GLY235 |
| A | HOH2203 |
| A | HOH2204 |
| A | HOH2205 |
| A | HOH2206 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 B1251 |
| Chain | Residue |
| B | GLY235 |
| B | HOH2175 |
| B | HOH2194 |
| B | HOH2195 |
| B | HOH2196 |
| B | SER213 |
| B | GLY234 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 C1251 |
| Chain | Residue |
| C | TRP170 |
| C | SER213 |
| C | GLY234 |
| C | GLY235 |
| C | HOH2195 |
| C | HOH2196 |
| C | HOH2197 |
| C | HOH2198 |
| site_id | AC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SO4 D1251 |
| Chain | Residue |
| D | SER213 |
| D | GLY234 |
| D | GLY235 |
| D | HOH2007 |
| D | HOH2205 |
| D | HOH2210 |
| D | HOH2211 |
| D | HOH2212 |
| D | HOH2213 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 E1251 |
| Chain | Residue |
| E | SER213 |
| E | GLY234 |
| E | GLY235 |
| E | HOH2176 |
| E | HOH2191 |
| E | HOH2202 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 F1251 |
| Chain | Residue |
| F | GLY234 |
| F | GLY235 |
| F | HOH2183 |
| F | HOH2184 |
| F | HOH2185 |
| F | HOH2186 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 G1251 |
| Chain | Residue |
| G | GLY235 |
| G | HOH2007 |
| G | HOH2116 |
| G | HOH2126 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 H1251 |
| Chain | Residue |
| H | SER213 |
| H | GLY234 |
| H | GLY235 |
| H | HOH2123 |
| H | HOH2132 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A1252 |
| Chain | Residue |
| A | THR31 |
| A | SER32 |
| A | ILE33 |
| A | HIS57 |
| A | LYS59 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 E1252 |
| Chain | Residue |
| E | THR31 |
| E | SER32 |
| E | ILE33 |
| E | HIS57 |
| E | HOH2205 |
| E | HOH2206 |
| site_id | BC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EPE C1252 |
| Chain | Residue |
| C | LYS155 |
| C | HOH2200 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EPE D1252 |
| Chain | Residue |
| D | ASN29 |
| D | THR31 |
| D | SER32 |
| D | ILE33 |
| D | HIS57 |
| D | LYS59 |
Functional Information from PROSITE/UniProt
| site_id | PS00171 |
| Number of Residues | 11 |
| Details | TIM_1 Triosephosphate isomerase active site. AYEPVWAIGTG |
| Chain | Residue | Details |
| A | ALA165-GLY175 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | ACT_SITE: Electrophile |
| Chain | Residue | Details |
| A | HIS95 | |
| B | HIS95 | |
| C | HIS95 | |
| D | HIS95 | |
| E | HIS95 | |
| F | HIS95 | |
| G | HIS95 | |
| H | HIS95 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | ACT_SITE: Proton acceptor |
| Chain | Residue | Details |
| A | GLU167 | |
| B | GLU167 | |
| C | GLU167 | |
| D | GLU167 | |
| E | GLU167 | |
| F | GLU167 | |
| G | GLU167 | |
| H | GLU167 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | ASN11 | |
| E | LYS13 | |
| F | ASN11 | |
| F | LYS13 | |
| G | ASN11 | |
| G | LYS13 | |
| H | ASN11 | |
| H | LYS13 | |
| A | LYS13 | |
| B | ASN11 | |
| B | LYS13 | |
| C | ASN11 | |
| C | LYS13 | |
| D | ASN11 | |
| D | LYS13 | |
| E | ASN11 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1hti |
| Chain | Residue | Details |
| A | LYS13 | |
| A | HIS95 | |
| A | ASN11 | |
| A | GLU167 |
| site_id | CSA2 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1hti |
| Chain | Residue | Details |
| B | LYS13 | |
| B | HIS95 | |
| B | ASN11 | |
| B | GLU167 | |
| B | GLY173 |
| site_id | CSA3 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1hti |
| Chain | Residue | Details |
| C | LYS13 | |
| C | HIS95 | |
| C | ASN11 | |
| C | GLU167 |
| site_id | CSA4 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1hti |
| Chain | Residue | Details |
| D | LYS13 | |
| D | HIS95 | |
| D | ASN11 | |
| D | GLU167 | |
| D | GLY173 |
| site_id | CSA5 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1hti |
| Chain | Residue | Details |
| E | LYS13 | |
| E | HIS95 | |
| E | ASN11 | |
| E | GLU167 |
| site_id | CSA6 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1hti |
| Chain | Residue | Details |
| F | LYS13 | |
| F | HIS95 | |
| F | ASN11 | |
| F | GLU167 |
| site_id | CSA7 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1hti |
| Chain | Residue | Details |
| G | LYS13 | |
| G | HIS95 | |
| G | ASN11 | |
| G | GLU167 | |
| G | GLY173 |
| site_id | CSA8 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1hti |
| Chain | Residue | Details |
| H | LYS13 | |
| H | HIS95 | |
| H | ASN11 | |
| H | GLU167 |
