2V0T
The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.690, 79.250, 174.000 |
| Unit cell angles | 90.00, 99.22, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.200 |
| R-factor | 0.181 |
| Rwork | 0.178 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ml1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.519 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.220 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.400 |
| Number of reflections | 103058 | |
| <I/σ(I)> | 13.4 | 3.4 |
| Completeness [%] | 98.4 | 88.3 |
| Redundancy | 4.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 8% ETHYLENE GLYCOL AND 10% PEG8000 |
