2V0T
The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-01-17 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.690, 79.250, 174.000 |
Unit cell angles | 90.00, 99.22, 90.00 |
Refinement procedure
Resolution | 19.810 - 2.200 |
R-factor | 0.181 |
Rwork | 0.178 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ml1 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.519 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.220 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.110 | 0.400 |
Number of reflections | 103058 | |
<I/σ(I)> | 13.4 | 3.4 |
Completeness [%] | 98.4 | 88.3 |
Redundancy | 4.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES PH 7.5, 8% ETHYLENE GLYCOL AND 10% PEG8000 |