2J7Q

Crystal structure of the ubiquitin-specific protease encoded by murine cytomegalovirus tegument protein M48 in complex with a ubquitin-based suicide substrate

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A1234

Chain	Residue
A	PRO41
A	ASP44
A	SER47
A	HOH2045
A	HOH2047
A	HOH2053

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B1076

Chain	Residue
D	LYS29
D	HOH2038
B	GLU18
B	HOH2015
B	HOH2016

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG D1076

Chain	Residue
B	LYS29
B	HOH2019
B	HOH2025
D	GLU18
D	HOH2026
D	HOH2027

---

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GVE A1235

Chain	Residue
A	GLY20
A	SER21
A	CYS23
A	TYR76
A	VAL140
A	HIS141
A	HOH2246
B	GLY75
D	GLN31

---

site_id	AC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PG4 B1077

Chain	Residue
A	GLU87
A	LEU119
A	ARG126
A	ALA151
A	THR152
A	CYS171
A	ASP172
A	ASP173
A	PHE174
A	THR175
A	HOH2110
A	HOH2163
B	HOH2082
B	HOH2083

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GVE C1235

Chain	Residue
C	GLY20
C	CYS23
C	TYR76
C	VAL140
C	PHE141
D	GLY75

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL C1234

Chain	Residue
C	PRO129
C	PRO131
C	VAL132
C	TYR133
C	VAL198
C	TYR221
C	HOH2277

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
B	LYS27-ASP52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
D	ARG54
D	ARG72
B	ARG54
B	ARG72

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Essential for function

Chain	Residue	Details
D	HIS68
B	HIS68

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291

Chain	Residue	Details
D	SER65
B	SER65

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
B	THR66
D	THR66

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
B	LYS6
D	LYS6

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
B	LYS63
D	LYS63

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
D	LYS11
D	LYS48
B	LYS11
B	LYS48

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
B	LYS27
D	LYS27

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
B	LYS29
D	LYS29

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
B	LYS33
D	LYS33

---