2J7Q
Crystal structure of the ubiquitin-specific protease encoded by murine cytomegalovirus tegument protein M48 in complex with a ubquitin-based suicide substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-31 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.907, 57.298, 67.279 |
| Unit cell angles | 73.37, 85.37, 88.54 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.21400 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.442 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.270 |
| Number of reflections | 52323 | |
| <I/σ(I)> | 8.3 | 3.5 |
| Completeness [%] | 97.4 | 84.2 |
| Redundancy | 4.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 200 MM MAGNESIUM FORMATE, 14% PEG 3350, pH 7.50 |
