1TSD

THYMIDYLATE SYNTHASE COMPLEX WITH 2'-DEOXYURIDINE 5'-MONOPHOSPHATE (DUMP) AND FOLATE ANALOG 1843U89

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003723	molecular_function	RNA binding
A	0004799	molecular_function	thymidylate synthase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006231	biological_process	dTMP biosynthetic process
A	0006235	biological_process	dTTP biosynthetic process
A	0006417	biological_process	regulation of translation
A	0008168	molecular_function	methyltransferase activity
A	0009165	biological_process	nucleotide biosynthetic process
A	0009314	biological_process	response to radiation
A	0016740	molecular_function	transferase activity
A	0016741	molecular_function	transferase activity, transferring one-carbon groups
A	0032259	biological_process	methylation
A	0042803	molecular_function	protein homodimerization activity
B	0000287	molecular_function	magnesium ion binding
B	0003723	molecular_function	RNA binding
B	0004799	molecular_function	thymidylate synthase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006231	biological_process	dTMP biosynthetic process
B	0006235	biological_process	dTTP biosynthetic process
B	0006417	biological_process	regulation of translation
B	0008168	molecular_function	methyltransferase activity
B	0009165	biological_process	nucleotide biosynthetic process
B	0009314	biological_process	response to radiation
B	0016740	molecular_function	transferase activity
B	0016741	molecular_function	transferase activity, transferring one-carbon groups
B	0032259	biological_process	methylation
B	0042803	molecular_function	protein homodimerization activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE BME A 265

Chain	Residue
A	LYS48
A	CYS50
A	HIS51
A	PHE176
A	F89268

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE BME A 266

Chain	Residue
A	LEU116
A	LYS120
A	GLN191
A	CYS192

---

site_id	AC3
Number of Residues	15
Details	BINDING SITE FOR RESIDUE UMP A 267

Chain	Residue
A	ARG21
A	CYS146
A	HIS147
A	GLN165
A	ARG166
A	SER167
A	ASP169
A	ASN177
A	HIS207
A	TYR209
A	F89268
A	HOH276
A	HOH418
B	ARG126
B	ARG127

---

site_id	AC4
Number of Residues	13
Details	BINDING SITE FOR RESIDUE F89 A 268

Chain	Residue
A	SER54
A	GLU58
A	THR78
A	ILE79
A	TRP80
A	TRP83
A	ASP169
A	GLY173
A	PHE176
A	TYR209
A	ALA263
A	BME265
A	UMP267

---

site_id	AC5
Number of Residues	16
Details	BINDING SITE FOR RESIDUE UMP B 265

Chain	Residue
A	ARG126
A	ARG127
B	ARG21
B	CYS146
B	HIS147
B	GLN165
B	ARG166
B	SER167
B	CYS168
B	ASP169
B	ASN177
B	HIS207
B	TYR209
B	F89266
B	HOH287
B	HOH300

---

site_id	AC6
Number of Residues	13
Details	BINDING SITE FOR RESIDUE F89 B 266

Chain	Residue
B	SER54
B	GLU58
B	VAL77
B	ILE79
B	TRP80
B	TRP83
B	ASP169
B	GLY173
B	PHE176
B	TYR209
B	ALA263
B	UMP265
B	HOH283

---

Functional Information from PROSITE/UniProt

site_id	PS00091
Number of Residues	29
Details	THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriIvsaWNvgeldkma.....LaPCHaffQFyV

Chain	Residue	Details
A	ARG126-VAL154

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"HAMAP-Rule","id":"MF_00008","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"2223754","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8973201","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9416600","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	14
Details	Binding site: {"description":"in other chain","evidences":[{"source":"PubMed","id":"2223754","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8973201","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1KCE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2TSC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"8312270","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1TYS","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"2223754","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8973201","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1KCE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2TSC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	6
Details	Annotated By Reference To The Literature 1b02

Chain	Residue	Details
A	HIS207
A	GLU58
A	ASP169
A	SER167
A	CYS146
A	ASP205

---

site_id	CSA2
Number of Residues	6
Details	Annotated By Reference To The Literature 1b02

Chain	Residue	Details
B	HIS207
B	GLU58
B	ASP169
B	SER167
B	CYS146
B	ASP205

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1b02

Chain	Residue	Details
A	SER180
A	ASN177
A	CYS146

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1b02

Chain	Residue	Details
B	SER180
B	ASN177
B	CYS146

---