1TSD
THYMIDYLATE SYNTHASE COMPLEX WITH 2'-DEOXYURIDINE 5'-MONOPHOSPHATE (DUMP) AND FOLATE ANALOG 1843U89
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Collection date | 1993-06-21 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 63 |
| Unit cell lengths | 127.520, 127.520, 68.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 1.950 |
| R-factor | 0.197 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.045 |
| Data reduction software | MADNES |
| Refinement software | GPRLSA |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 1.950 |
| Rmerge | 0.087 |
| Number of reflections | 48550 |
| Completeness [%] | 98.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | E. coli | 31 (mg/ml) | |
| 2 | 1 | drop | dUMP | 5 (mM) | |
| 3 | 1 | drop | 1843U89 | 0.5 (mM) | |
| 4 | 1 | drop | phosphate | 20 (mM) | |
| 5 | 1 | drop | 2-mercaptoethanol | 20 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 2.6 (M) |
