1NVU
Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| Q | 0003924 | molecular_function | GTPase activity |
| Q | 0005525 | molecular_function | GTP binding |
| Q | 0007165 | biological_process | signal transduction |
| Q | 0016020 | cellular_component | membrane |
| R | 0003924 | molecular_function | GTPase activity |
| R | 0005525 | molecular_function | GTP binding |
| R | 0007165 | biological_process | signal transduction |
| R | 0016020 | cellular_component | membrane |
| S | 0005085 | molecular_function | guanyl-nucleotide exchange factor activity |
| S | 0007264 | biological_process | small GTPase-mediated signal transduction |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG Q 1002 |
| Chain | Residue |
| Q | SER17 |
| Q | THR35 |
| Q | GTP1001 |
| Q | HOH1008 |
| Q | HOH1011 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PO4 R 1003 |
| Chain | Residue |
| R | SER17 |
| R | GLY59 |
| R | GLU62 |
| R | HOH1074 |
| R | GLY13 |
| R | VAL14 |
| R | GLY15 |
| R | LYS16 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PO4 S 1047 |
| Chain | Residue |
| S | HOH127 |
| S | HOH363 |
| S | ARG625 |
| S | HIS695 |
| S | HIS699 |
| S | HIS700 |
| site_id | AC4 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PO4 S 1048 |
| Chain | Residue |
| R | GLU63 |
| R | SER127 |
| R | ARG128 |
| R | GLN131 |
| R | HOH1028 |
| S | HOH372 |
| S | HOH384 |
| S | LYS814 |
| S | GLU815 |
| S | ARG826 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO4 S 1049 |
| Chain | Residue |
| S | HOH115 |
| S | ARG660 |
| S | TYR709 |
| S | GLU716 |
| S | HIS951 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PO4 Q 1007 |
| Chain | Residue |
| Q | GLU153 |
| Q | ASP154 |
| Q | TYR157 |
| Q | ARG161 |
| site_id | AC7 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE GTP Q 1001 |
| Chain | Residue |
| Q | GLY12 |
| Q | GLY13 |
| Q | VAL14 |
| Q | GLY15 |
| Q | LYS16 |
| Q | SER17 |
| Q | ALA18 |
| Q | PHE28 |
| Q | VAL29 |
| Q | ASP30 |
| Q | TYR32 |
| Q | PRO34 |
| Q | THR35 |
| Q | GLY60 |
| Q | GLU62 |
| Q | ASN116 |
| Q | LYS117 |
| Q | ASP119 |
| Q | LEU120 |
| Q | SER145 |
| Q | ALA146 |
| Q | LYS147 |
| Q | MG1002 |
| Q | HOH1008 |
| Q | HOH1011 |
| Q | HOH1019 |
Functional Information from PROSITE/UniProt
| site_id | PS00720 |
| Number of Residues | 34 |
| Details | RASGEF Ras Guanine-nucleotide exchange factors domain signature. VPFfGiyLtNIlkteegnpevlkrhgkel.................INFsK |
| Chain | Residue | Details |
| S | VAL927-LYS960 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 16 |
| Details | Motif: {"description":"Effector region"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 34 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"16698776","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"35522713","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N-acetylmethionine; in GTPase HRas; alternate","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N-acetylthreonine; in GTPase HRas, N-terminally processed","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"PubMed","id":"9020151","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL","evidences":[{"source":"PubMed","id":"19744486","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8626575","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8626586","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9632667","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 144 |
| Details | Domain: {"description":"N-terminal Ras-GEF","evidences":[{"source":"PROSITE-ProRule","id":"PRU00135","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 239 |
| Details | Domain: {"description":"Ras-GEF","evidences":[{"source":"PROSITE-ProRule","id":"PRU00168","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| Q | GLN61 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| R | GLN61 |
