1NVU

Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS

Functional Information from GO Data

Chain	GOid	namespace	contents
Q	0003924	molecular_function	GTPase activity
Q	0005525	molecular_function	GTP binding
Q	0007165	biological_process	signal transduction
Q	0016020	cellular_component	membrane
R	0003924	molecular_function	GTPase activity
R	0005525	molecular_function	GTP binding
R	0007165	biological_process	signal transduction
R	0016020	cellular_component	membrane
S	0005085	molecular_function	guanyl-nucleotide exchange factor activity
S	0007264	biological_process	small GTPase-mediated signal transduction

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG Q 1002

Chain	Residue
Q	SER17
Q	THR35
Q	GTP1001
Q	HOH1008
Q	HOH1011

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site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PO4 R 1003

Chain	Residue
R	SER17
R	GLY59
R	GLU62
R	HOH1074
R	GLY13
R	VAL14
R	GLY15
R	LYS16

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 S 1047

Chain	Residue
S	HOH127
S	HOH363
S	ARG625
S	HIS695
S	HIS699
S	HIS700

---

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 S 1048

Chain	Residue
R	GLU63
R	SER127
R	ARG128
R	GLN131
R	HOH1028
S	HOH372
S	HOH384
S	LYS814
S	GLU815
S	ARG826

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 S 1049

Chain	Residue
S	HOH115
S	ARG660
S	TYR709
S	GLU716
S	HIS951

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PO4 Q 1007

Chain	Residue
Q	GLU153
Q	ASP154
Q	TYR157
Q	ARG161

---

site_id	AC7
Number of Residues	26
Details	BINDING SITE FOR RESIDUE GTP Q 1001

Chain	Residue
Q	GLY12
Q	GLY13
Q	VAL14
Q	GLY15
Q	LYS16
Q	SER17
Q	ALA18
Q	PHE28
Q	VAL29
Q	ASP30
Q	TYR32
Q	PRO34
Q	THR35
Q	GLY60
Q	GLU62
Q	ASN116
Q	LYS117
Q	ASP119
Q	LEU120
Q	SER145
Q	ALA146
Q	LYS147
Q	MG1002
Q	HOH1008
Q	HOH1011
Q	HOH1019

---

Functional Information from PROSITE/UniProt

site_id	PS00720
Number of Residues	34
Details	RASGEF Ras Guanine-nucleotide exchange factors domain signature. VPFfGiyLtNIlkteegnpevlkrhgkel.................INFsK

Chain	Residue	Details
S	VAL927-LYS960

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	BINDING: BINDING => ECO:0000269|PubMed:16698776

Chain	Residue	Details
Q	GLY13
R	SER145
Q	VAL29
Q	GLY59
Q	ASN116
Q	SER145
R	GLY13
R	VAL29
R	GLY59
R	ASN116

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N-acetylmethionine; in GTPase HRas; alternate => ECO:0000269|Ref.12

Chain	Residue	Details
Q	MET1
R	MET1

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N-acetylthreonine; in GTPase HRas, N-terminally processed => ECO:0000269|Ref.12

Chain	Residue	Details
Q	THR2
R	THR2

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:9020151

Chain	Residue	Details
Q	CYS118
R	CYS118

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL => ECO:0000269|PubMed:19744486, ECO:0000269|PubMed:8626575, ECO:0000269|PubMed:8626586, ECO:0000269|PubMed:9632667

Chain	Residue	Details
Q	THR35
R	THR35

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