1NVU
Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-03 |
| Detector | SBC-2 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 183.312, 183.312, 178.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.000 * - 2.210* |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.21200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 21.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 * | 0.512 * |
| Total number of observations | 758703 * | |
| Number of reflections | 75882 | |
| <I/σ(I)> | 18.7 | 3.3 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 10 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | reservoir | phosphate | 1.2-1.5 (M) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
