8X3S
Crystal structure of human WDR5 in complex with PTEN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.460, 63.750, 92.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.190 - 1.870 |
Rwork | 0.224 |
R-free | 0.25860 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.190 | 1.910 |
High resolution limit [Å] | 1.870 | 1.870 |
Number of reflections | 22670 | 1831 |
<I/σ(I)> | 10.5 | |
Completeness [%] | 97.0 | |
Redundancy | 1.8 | |
CC(1/2) | 0.994 | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M lithium sulfate monohydrate, 0.1 M HEPES at pH 7.5, 25% w/v polyethylene glycol 3350 |