8TRT
Structure of the EphA2 CRD bound to FabS1CE_C1, monoclinic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.710, 182.135, 74.380 |
Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
Resolution | 68.780 - 3.000 |
R-factor | 0.1966 |
Rwork | 0.194 |
R-free | 0.24420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8trs |
RMSD bond length | 0.011 |
RMSD bond angle | 1.211 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.780 | 3.040 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.195 | 0.874 |
Rmeas | 0.211 | 0.959 |
Rpim | 0.079 | 0.384 |
Number of reflections | 32852 | 4372 |
<I/σ(I)> | 5.7 | 1.9 |
Completeness [%] | 97.0 | 97 |
Redundancy | 6.6 | 5.8 |
CC(1/2) | 0.989 | 0.783 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 200 mM sodium chloride, 100 mM Bis-Tris, 20% PEG3350 |