8SZY

Crystal Structure of Heterotrimeric Anti-TIGIT Fabs in complex with human TIGIT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	45.850, 216.910, 109.450
Unit cell angles	90.00, 95.97, 90.00

Refinement procedure

Resolution	45.600 - 2.310
R-factor	0.229
Rwork	0.227
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.881
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	108.856	2.479
High resolution limit [Å]	2.306	2.306
Rmerge	0.140	0.694
Rmeas	0.168	0.854
Rpim	0.091	0.491
Number of reflections	65410	3271
<I/σ(I)>	7.5	1.5
Completeness [%]	70.2
Redundancy	3.3
CC(1/2)	0.985	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	0.2 M sodium phosphate monobasic monohydrate, 20% w/v Polyethylene glycol 1,000