8SZY
Crystal Structure of Heterotrimeric Anti-TIGIT Fabs in complex with human TIGIT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.850, 216.910, 109.450 |
| Unit cell angles | 90.00, 95.97, 90.00 |
Refinement procedure
| Resolution | 45.600 - 2.310 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.881 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.856 | 2.479 |
| High resolution limit [Å] | 2.306 | 2.306 |
| Rmerge | 0.140 | 0.694 |
| Rmeas | 0.168 | 0.854 |
| Rpim | 0.091 | 0.491 |
| Number of reflections | 65410 | 3271 |
| <I/σ(I)> | 7.5 | 1.5 |
| Completeness [%] | 70.2 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.985 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.2 M sodium phosphate monobasic monohydrate, 20% w/v Polyethylene glycol 1,000 |
