8SIH
Crystal Structure of PRMT4 with Compound YD1-289
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.582, 86.582, 136.026 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.380 - 2.350 |
| R-factor | 0.19723 |
| Rwork | 0.196 |
| R-free | 0.24587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.054 | 0.027 | 0.987 |
| Rmeas | 0.057 | 0.029 | 1.055 |
| Rpim | 0.020 | 0.010 | 0.367 |
| Total number of observations | 206006 | ||
| Number of reflections | 25134 | 1393 | 1235 |
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 99.9 | 99.9 | 100 |
| Redundancy | 8.2 | 7.5 | 8.2 |
| CC(1/2) | 0.998 | 0.999 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 3.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0 |
