8RQC
Crystal structure of CRBN-midi in complex with mezigdomide and IKZF1 ZF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.957 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.585, 142.842, 56.693 |
| Unit cell angles | 90.00, 112.34, 90.00 |
Refinement procedure
| Resolution | 52.440 - 2.150 |
| R-factor | 0.2619 |
| Rwork | 0.261 |
| R-free | 0.28590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.461 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.440 | 2.227 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.200 | 3.696 |
| Rmeas | 0.216 | 3.984 |
| Rpim | 0.081 | 1.477 |
| Number of reflections | 42776 | 4266 |
| <I/σ(I)> | 6.12 | 0.32 |
| Completeness [%] | 97.6 | 80.1 |
| Redundancy | 7.1 | 7.2 |
| CC(1/2) | 0.996 | 0.343 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium sulphate, 25% (w/v) PEG 3350, and 0.1 M HEPES pH 7.5 |
