8RQC

Crystal structure of CRBN-midi in complex with mezigdomide and IKZF1 ZF2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.957
Spacegroup name	P 1 21 1
Unit cell lengths	53.585, 142.842, 56.693
Unit cell angles	90.00, 112.34, 90.00

Refinement procedure

Resolution	52.440 - 2.150
R-factor	0.2619
Rwork	0.261
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.461
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.440	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.200	3.696
Rmeas	0.216	3.984
Rpim	0.081	1.477
Number of reflections	42776	4266
<I/σ(I)>	6.12	0.32
Completeness [%]	97.6	80.1
Redundancy	7.1	7.2
CC(1/2)	0.996	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulphate, 25% (w/v) PEG 3350, and 0.1 M HEPES pH 7.5