8QZZ

Crystal structure of human eIF2 alpha-gamma complexed with PPP1R15A_420-452

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-29
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.6199
Spacegroup name	P 41 21 2
Unit cell lengths	120.594, 120.594, 158.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.150 - 3.350
R-factor	0.24368
Rwork	0.241
R-free	0.29915
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.911
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.170	3.620
High resolution limit [Å]	3.350	3.350
Rmerge	0.271	2.618
Rmeas	0.280	2.714
Rpim	0.113	1.109
Number of reflections	17421	3497
<I/σ(I)>	7.2
Completeness [%]	99.9
Redundancy	12.3
CC(1/2)	0.996	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	11-12% PEG 6000, 0.1M Tris-HCl pH8.5 supplemented with amino acids