8QZ2
Crystal structure of human two pore domain potassium ion channel TREK-2 (K2P10.1) in complex with an inhibitory nanobody (Nb61)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.538, 111.698, 289.208 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.100 - 3.500 |
| R-factor | 0.2809 |
| Rwork | 0.280 |
| R-free | 0.30820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.680 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.842 | 3.577 |
| High resolution limit [Å] | 3.499 | 3.499 |
| Rmeas | 0.540 | 1.997 |
| Rpim | 0.223 | 0.799 |
| Number of reflections | 11445 | 572 |
| <I/σ(I)> | 3.7 | 1.2 |
| Completeness [%] | 95.0 | 79.7 |
| Redundancy | 5.6 | 6.1 |
| CC(1/2) | 0.997 | 0.488 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.5 | 293 | MAG7.9, 0.1 M Na acetate pH 4.5, 0.3 M KCl, 23% v/v polyethylene glycol 400 |
