8P2L
A CHIMERA construct containing human SARM1 ARM and SAM domains and C. elegans TIR domain.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.68 |
| Number of Particle | 298564 |
| Software | UCSF ChimeraX,ISOLDE,cryoSPARC,EPU,Coot,REFMAC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
