8OW2

Crystal structure of the p110alpha catalytic subunit from homo sapiens in complex with activator 1938

Replaces:  8BFV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	58.720, 134.880, 144.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.460 - 2.570
R-factor	0.2158
Rwork	0.213
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.21rc1_4924)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	135.000	2.610
High resolution limit [Å]	2.570	2.570
Rmeas	0.140	2.600
Number of reflections	37543	1832
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	8.7
CC(1/2)	0.990	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	12.5% (w/v) PEG 4K, 20% (v/v) 1,2,6-hexanetriol, 50 mM Polyamines, 0.1 M MOPSO/bis-tris pH 6.5