8I5W
Crystal structure of the DHR-2 domain of DOCK10 in complex with Rac1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-12-13 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.280, 106.860, 187.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.778 - 2.432 |
| R-factor | 0.2249 |
| Rwork | 0.220 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8i5f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.851 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.580 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.996 | |
| Rmeas | 0.331 | |
| Number of reflections | 23123 | 3630 |
| <I/σ(I)> | 8.69 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | ammonium sulfate, sodium cacodylate, PEG 8000, glycerol |
