8H75
FGFR2 in complex with YJ001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.022, 198.595, 67.203 |
| Unit cell angles | 90.00, 92.94, 90.00 |
Refinement procedure
| Resolution | 27.800 - 3.750 |
| R-factor | 0.2164 |
| Rwork | 0.214 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oec |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.777 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.740 | 3.740 |
| Rpim | 0.092 | 0.346 |
| Number of reflections | 16042 | 803 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 93.3 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2M (NH4)2SO4, 0.1M Bis-Tris pH6.0, 25% PEG3350. |
