8GU7
Selective targeting of the Beclin 2-Atg14L coiled coil complex by stapled peptides promotes autophagy and endolysosomal trafficking of GPCRs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 289 |
Detector technology | CCD |
Collection date | 2017-04-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.500, 72.500, 68.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.110 - 2.600 |
R-factor | 0.2183 |
Rwork | 0.217 |
R-free | 0.23970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8gt9 |
Data reduction software | SCALEIT |
Data scaling software | SCALA |
Phasing software | Arcimboldo |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.260 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 6736 | 951 |
<I/σ(I)> | 14.1 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 12.2 | |
CC(1/2) | 0.995 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 1.8 M Ammonium citrate tribasic, pH 7.0 |