8GT9

Selective targeting of the Beclin 2-Atg14L coiled coil complex by stapled peptides promotes autophagy and endolysosomal trafficking of GPCRs

Summary for 8GT9

• Entry DOI: 10.2210/pdb8gt9/pdb
• Descriptor: Beclin-2 (2 entities in total)
• Functional Keywords: pi3kc3 complex, beclin 2, autophagy, structural protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 4
• Total formula weight: 43883.68

Authors

Qiu, X.,Zhao, Y. (deposition date: 2022-09-07, release date: 2023-07-26, Last modification date: 2023-10-11)

Primary citation

Qiu, X.,Li, N.,Yang, Q.,Wu, S.,Li, X.,Pan, X.,Yamamoto, S.,Zhang, X.,Zeng, J.,Liao, J.,He, C.,Wang, R.,Zhao, Y.
The potent BECN2-ATG14 coiled-coil interaction is selectively critical for endolysosomal degradation of GPRASP1/GASP1-associated GPCRs.
Autophagy, 19:2884-2898, 2023

PubMed Abstract: AMBRA1 autophagy and beclin 1 regulator 1; ATG14 autophagy related 14; ATG5 autophagy related 5; ATG7 autophagy related 7; BECN1 beclin 1; BECN2 beclin 2; CC coiled-coil; CQ chloroquine CNR1/CB1R cannabinoid receptor 1 DAPI 4',6-diamidino-2-phenylindole; dCCD delete CCD; DRD2/D2R dopamine receptor D2 GPRASP1/GASP1 G protein-coupled receptor associated sorting protein 1 GPCR G-protein coupled receptor; ITC isothermal titration calorimetry; IP immunoprecipitation; KD knockdown; KO knockout; MAP1LC3/LC3 microtubule associated protein 1 light chain 3; NRBF2 nuclear receptor binding factor 2; OPRD1/DOR opioid receptor delta 1 PIK3C3/VPS34 phosphatidylinositol 3-kinase catalytic subunit type 3; PIK3R4/VPS15 phosphoinositide-3-kinase regulatory subunit 4; PtdIns3K class III phosphatidylinositol 3-kinase; PtdIns3P phosphatidylinositol-3-phosphate; RUBCN rubicon autophagy regulator; SQSTM1/p62 sequestosome 1; UVRAG UV radiation resistance associated; VPS vacuolar protein sorting; WT wild type.

PubMed: 37409929
DOI: 10.1080/15548627.2023.2233872

Experimental method

X-RAY DIFFRACTION (2 Å)