8GU7

Selective targeting of the Beclin 2-Atg14L coiled coil complex by stapled peptides promotes autophagy and endolysosomal trafficking of GPCRs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beclin 1-associated autophagy-related key regulator	polymer	60	7021.2	2	UniProt (Q8CDJ3)	Mus musculus (house mouse)	Barkor,Autophagy-related protein 14-like protein,Atg14L
2	C, D (C, D)	Beclin-2	polymer	96	10970.9	2	UniProt (A8MW95) Pfam (PF17675)	Homo sapiens (human)	Beclin-1 autophagy-related pseudogene 1,Beclin-1-like protein 1
3	E, F, G (A, B, D)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, B: 5 - 58 (UniProt: Q8CDJ3)

PDB	External Database	Details
Gly -1	-	expression tag
Pro 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Leu 20	Cys 116	engineered mutation
Ser 32	Cys 128	engineered mutation
Leu 34	Gly 130	engineered mutation

C, D: 5 - 96 (UniProt: A8MW95)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Leu 91	Arg 243	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	35984.3
All*	Total formula weight	35984.3

*Water molecules are not included.