8EJM

Crystal structure of human DEAH-box helicase DHX15 in complex with SUGP1 G-patch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	82.300, 90.956, 213.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.670 - 1.800
R-factor	0.1905
Rwork	0.189
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sh6
RMSD bond length	0.013
RMSD bond angle	1.154
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.700	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.066	1.324
Number of reflections	74658	11895
<I/σ(I)>	13.2
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.999	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350, ammonium sulfate, HEPES buffer