8E9E

Rat protein farnesyltransferase in complex with FPP and inhibitor 2f

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.116
Spacegroup name	P 61
Unit cell lengths	169.898, 169.898, 69.339
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.380 - 2.844
R-factor	0.1772
Rwork	0.176
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e33
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.380	2.946
High resolution limit [Å]	2.844	2.844
Rmerge	0.156	0.744
Rmeas	0.171	0.818
Rpim	0.067	0.323
Number of reflections	26864	2626
<I/σ(I)>	10.49	1.98
Completeness [%]	99.0	97.8
Redundancy	5.8	5.4
CC(1/2)	0.991	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	290	50-150mM Na Acetate pH 5.5, 1-2.5% PEG8000