8E0F

Human Adenosine Deaminase Acting on dsRNA (ADAR2-RD) bound to dsRNA containing a G-G pair adjacent to the target site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	171.518, 63.389, 142.135
Unit cell angles	90.00, 117.69, 90.00

Refinement procedure

Resolution	65.730 - 2.700
R-factor	0.1952
Rwork	0.193
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vff
Data reduction software	XDS
Data scaling software	Aimless (2.7.28)
Phasing software	PHENIX (1.18)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.850	125.850	2.820
High resolution limit [Å]	2.700	9.350	2.700
Rmerge	0.075	0.039	0.728
Rmeas	0.091	0.047	0.888
Rpim	0.051	0.026	0.500
Total number of observations	112747
Number of reflections	36652	910	4467
<I/σ(I)>	9.6
Completeness [%]	97.8	94.2	98.6
Redundancy	3.1	3.1	3
CC(1/2)	0.995	0.995	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	50 mM MOPS pH 7.0, 17% (w/v) PEG 4000, 200 mM NaCl