8DPV

The crystal structure of Interleukin-11, W147A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 2
Unit cell lengths	38.505, 133.996, 27.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.010 - 1.480
R-factor	0.1842
Rwork	0.182
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mhl
RMSD bond length	0.013
RMSD bond angle	1.284
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.670	1.510
High resolution limit [Å]	1.480	1.480
Rmeas	0.089	2.003
Rpim	0.034	0.743
Number of reflections	24354	1182
<I/σ(I)>	12.9	1.3
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.999	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	PEG 3350, 200 mM ammonium sulphate, 100 mM Tris pH 8.5