8D5B
Crystal structure of human METTL1 in complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 61 |
Unit cell lengths | 86.034, 86.034, 65.483 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.250 - 1.930 |
R-factor | 0.1823 |
Rwork | 0.181 |
R-free | 0.21220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ckk |
RMSD bond length | 0.006 |
RMSD bond angle | 0.951 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.250 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.242 | 3.220 |
Rpim | 0.057 | 1.030 |
Number of reflections | 20856 | 1034 |
<I/σ(I)> | 16.24 | 1.3 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 18.1 | 10.5 |
CC(1/2) | 0.996 | 0.450 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 50 mM Sodium cacodylate pH 5.7, 1.5 M Lithium sulfate, 10 mM Magnesium sulfate |