8CYH
Novel Anti-Mesothelin Antibodies Enable Crystallography of the Intact Mesothelin Ectodo- main and Engineering of Potent, T cell-engaging Bispecific Therapeutics
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.112, 124.862, 302.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.380 - 3.380 |
| R-factor | 0.2315 |
| Rwork | 0.230 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f3f |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.140 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.410 |
| High resolution limit [Å] | 3.350 | 9.080 | 3.350 |
| Rmerge | 0.149 | 0.049 | 0.934 |
| Rmeas | 0.164 | 0.055 | 1.035 |
| Rpim | 0.068 | 0.025 | 0.438 |
| Total number of observations | 101701 | ||
| Number of reflections | 17506 | 953 | 842 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 98.7 | 96.9 | 96.1 |
| Redundancy | 5.8 | 4.7 | 5.6 |
| CC(1/2) | 0.996 | 0.598 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Tris (8.0), sodium citrate, Peg3350 |
