8CYH

Novel Anti-Mesothelin Antibodies Enable Crystallography of the Intact Mesothelin Ectodo- main and Engineering of Potent, T cell-engaging Bispecific Therapeutics

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	A12 antibody light chain	polymer	214	23325.7	1		Homo sapiens (Human)
2	B (H)	A12 antibody heavy chain	polymer	222	23308.2	1		Homo sapiens (Human)
3	C (M)	Mesothelin, cleaved form	polymer	327	36963.2	1	UniProt (Q13421) Pfam (PF06060)	Homo sapiens (human)
4	D, E (M)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

M: 296 - 605 (UniProt: Q13421)

PDB	External Database	Details
Gly 606	-	expression tag
Ser 607	-	expression tag
Gly 608	-	expression tag
Leu 609	-	expression tag
Asn 610	-	expression tag
Asp 611	-	expression tag
Ile 612	-	expression tag
Phe 613	-	expression tag
Glu 614	-	expression tag
Ala 615	-	expression tag
Gln 616	-	expression tag
Lys 617	-	expression tag
Ile 618	-	expression tag
Glu 619	-	expression tag
Trp 620	-	expression tag
His 621	-	expression tag
Glu 622	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	83597.1
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	84039.5

*Water molecules are not included.