8CX3
Crystal structure of full-length mesothelin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.89197 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 197.171, 279.324, 170.046 |
| Unit cell angles | 90.00, 125.09, 90.00 |
Refinement procedure
| Resolution | 49.292 - 3.609 |
| Rwork | 0.291 |
| R-free | 0.31050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ued |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.797 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.123 | 0.480 |
| Number of reflections | 78378 | 6674 |
| <I/σ(I)> | 4.87 | |
| Completeness [%] | 91.4 | 78.1 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 289 | 0.9-1.1 M sodium/potassium phosphate, pH 8.2 |
