8BTL

Crystal structure of a complex between the E2 conjugating enzyme UBE2A and the E3 ligase module from UBR4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9118
Spacegroup name	P 43 21 2
Unit cell lengths	89.157, 89.157, 263.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.830 - 3.200
R-factor	0.23
Rwork	0.228
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.440
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.830	3.315
High resolution limit [Å]	3.200	3.200
Number of reflections	18375	1813
<I/σ(I)>	8.04	1.35
Completeness [%]	99.6	99.5
Redundancy	12.5	13.1
CC(1/2)	0.997	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	277	0.1 M Bis-Tris, pH 6.4, 15% PEG10000, 0.2 M Ammonium acetate